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Plasmid#145428PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
LifeAct-mScarletI pDRF1-GW
Plasmid#168085PurposeExpresses Lifeact-mScarletI to visualize actin in yeastDepositorInsertLifeAct-mScarletI
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-2TAG
Plasmid#158138PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)DepositorInsertBFP-GFP fragment
UseTagsExpressionYeastMutationCloned in a TAG codon at the first serine residue…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d_GgPCFT
Plasmid#165414Purposeexpression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.DepositorInsertGgPCFT (SLC46A1 Synthetic, Chicken)
UseTagsGFP-His8ExpressionYeastMutationPromoterGAL1Available sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN069
Plasmid#166728PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-Mxi1
UseCRISPRTagsMxi1ExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046319
Plasmid#153866PurposeYeast expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046292
Plasmid#149062PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;StrepIIExpressionYeastMutationwtPromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPTK016-3-RFP_Intra
Plasmid#84975PurposeType 3, Red fluorescent proteinDepositorInsertRed fluorescent protein
UseSynthetic BiologyTagsExpressionYeastMutationBsaI site removed (1677 c to g and 1794 a to g)PromoterNonAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046249
Plasmid#148991PurposeYeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUB23-K-beta-gal
Plasmid#8819DepositorInsertbeta-gal
UseTagsUbiquitinExpressionYeastMutationK (lysine) at amino terminus of beta-galPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
721-2µ-staHIS3
Plasmid#40611DepositorInsertHIS3 (HIS3 Budding Yeast)
UseTagsHAExpressionYeastMutationPromoterTDH3Available sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXP522
Plasmid#26847DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
RPL25NLS-GFP-PRA (102)
Plasmid#24041DepositorInsertRPL25 (RPL25 Budding Yeast)
UseTagsEGFP and PRAExpressionYeastMutationOnly bp 1-135 (aa 1-45) of RPL25PromoterAvailable sinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pK218TK620T
Plasmid#1192DepositorInsertHsp104 (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationchanged lysine 218 to threonine and lysine 620 to…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRS314-hUI
Plasmid#11006DepositorInserthUI recombination system
UseTagsExpressionBacterial and YeastMutationInverted repeat recombination system. Recombinati…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pWS788
Plasmid#123059Purpose_-factor proteaseDepositorInsertBar1 [3]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a- mECitrine-kanMX6
Plasmid#105150PurposeYeast gene targetingDepositorInsertmECitrine-kanMX6
UseTagsmECitrineExpressionBacterial and YeastMutationF64L, Q69M, A206KPromoterAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046230
Plasmid#145454PurposeYeast Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpTEF1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pAG424-ccdB
Plasmid#70124PurposeYeast Gateway TRP1 2micron vector, no promoter (GPD1 promoter removed)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterNoneAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only