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Plasmid#15569DepositorInserthtt 104Q Delta Pro (HTT Human)
UseTagsGFPExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJT131_GalL_cCDA1-NGBE3
Plasmid#145095PurposeExpressing base editor cCDA1-NGBE3 in yeast cellsDepositorInsertcCDA1-NGBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKT0174
Plasmid#8720DepositorInsertyECFP
UseTagsCodon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N144I, M153T, V163APromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
POM42-mCitrine
Plasmid#69854PurposeN-terminal tagging with mCitrine using URA3 as a selection marker in S. cerevisiae.DepositorInsertmCitrine minus start and stop codon
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-3FLAG
Plasmid#19338DepositorInsert3FLAG
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCfB3047(gRNA XII-1)
Plasmid#73289PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
U6-U57C
Plasmid#169928PurposeWT U6 with a U57C mutationDepositorInsertU6 U57C
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM2_12
Plasmid#183061PurposeControl for biosensor in pMM2_9: BM3R1-HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locusDepositorInsertsmCherry
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastMutationPromoterHAA1 and Synthetic promoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM2_4
Plasmid#183057PurposeHAA1-mTurquoise2 under HAA1p and mRuby2 under YGP1p with flanks for genome integration into HO locusDepositorInsertsmRuby2
HAA1-mTurquoise2
UseSynthetic BiologyTagsmRuby2 and mTurquoise2ExpressionYeastMutationPromoterHAA1 and YGP1Available sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM2_10
Plasmid#183059PurposeControl for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locusDepositorInsertsmCherry
HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastMutationPromoterHAA1 and Synthetic promoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM2_9
Plasmid#183058PurposeBiosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under HAA1p and mCherry under BM3R1ENO1cp with flanks for genome integration in toHO locusDepositorInsertsmCherry
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastMutationPromoterHAA1 and Synthetic promoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM2_11
Plasmid#183060PurposeControl for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locusDepositorInsertsmCherry
HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastMutationPromoterHAA1 and Synthetic promoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG423GPD-ccdB-DsRed
Plasmid#14366DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 + VP2C-GFP
Plasmid#166676PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertsVP2C-GFP
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV0586
Plasmid#133473PurposeIntegration into fission yeast genome at lys3 locus with bsdMX selection cassetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM12
Plasmid#133638PurposeIntegration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorInsertpCUP1-GFP-Tlg2 (TLG2 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yoeGFP-T2A-mCherry
Plasmid#118446PurposeProduces equimolar expression of yeast codon-optimized eGFP and mCherryDepositorInsertyoeGFP-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
P413TEF-PYK1
Plasmid#34605DepositorInsertPYK1
UseTagsExpressionYeastMutationPromoterTEFAvailable sinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
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LHP424-YCplac33-STE2-WT
Plasmid#32171DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterSTE2Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only