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pCSN074
Plasmid#166731PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS
UseCRISPRTagsSV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6-mTFP1-His3MX6
Plasmid#69850PurposeC-terminal tagging with mTFP1 using His3MX6 as a selection marker in S. cerevisiae.DepositorInsertmTFP1
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-SweetTrac1
Plasmid#169083PurposeExpression of SweetTrac1 biosensor in yeastDepositorInsertSweetTrac1
UseTagsExpressionYeastMutationPromoterPMA1Available sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG303GAL-EGFP-ccdB
Plasmid#14301DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
GTH-y
Plasmid#81105PurposeGene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1-YFP
Plasmid#18685DepositorInsertRnq1 (RNQ1 Budding Yeast)
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
E2-Crimson-FKBP(L)
Plasmid#85001PurposeExpresses E2-Crimson-FKBP(L)DepositorInsertE2-Crimson-FKBP(L)
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-STV1-iGFP
Plasmid#177111PurposeExpresses Stv1-iGFP in yeast cellsDepositorInsertSTV1 (STV1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
p416GAL1-Zipp.-L-IFP-F[1] (Yeast)
Plasmid#52895PurposePlasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[1]
UseTagsL-IFP-F[1]ExpressionYeastMutationPromoterGAL1Available sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046692
Plasmid#149195PurposeYeast Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046381
Plasmid#149010PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-link-mDsRed-SpHis5
Plasmid#44639PurposeYeast tagging vector to create gene fusion with mDsRed with SpHis5 selectionDepositorInsertmDsRed
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSLeu2d-BISopt
Plasmid#35524DepositorInsertBisabolene Synthase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterP GAL 1Available sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
BB3aN_14*
Plasmid#98548Purposeempty BB3 for insertion and overexpression of 1 gene in P. pastorisDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEmerald-Kan
Plasmid#44946PurposeYeast tagging vector to create gene fusion with yeast optimized Emerald with Kan selectionDepositorInsertyoEmerald
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Ptdh3-CAD-Ttdh1
Plasmid#127727PurposeYeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPtdh3Available sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRG714_lexO-AscI_LexA-TF_URA3MX
Plasmid#154822PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTCW008
Plasmid#83526Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoterDepositorInsertpFUS1J2-mfalpha2
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pICE ADE2-HphMx
Plasmid#122895PurposeADE2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceOct. 13, 2019AvailabilityAcademic Institutions and Nonprofits only
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