-
Plasmid#127609Purposehigh-copy URA-selectable plasmid, Mut ACT1 intron (stable) inserted into URA3DepositorInsertStable ACT1 intron (Mut)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
GTT-g
Plasmid#81109PurposeGene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG413GPD-ccdB-EYFP
Plasmid#14214DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN
Plasmid#99049PurposeTranscription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domainDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJY3948
Plasmid#113338Purpose6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expressionDepositorInsert2-pyrone synthase, codon optimized for Y. lipolytica
UseTags6XHisExpressionYeastMutationPlease see depositor commentsPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGK415-ÂrtetTAK8N, L131L
Plasmid#165969PurposeExpresses rTetTA (K8N, L131L) mutantDepositorInserttetR
UseSynthetic BiologyTagsthree copies of VP16 (VP48)ExpressionYeastMutationPromoterpPGK1Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNS1
Plasmid#131769PurposePADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sitesDepositorInsertFUSN_Fusion Red_Cry2WT
UseIntegration into δ-sitesTagsExpressionYeastMutationPromoterPADH1Available sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pBS10
Plasmid#83795PurposeUseful for tagging yeast proteins with C-terminal Cerulean tag; hygromycin-B selectionDepositorInsertCerulean
UseTagsCeruleanExpressionYeastMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
M4758 KanMX::URA3 Disruptor Converter
Plasmid#51688PurposeYeast marker swap plasmid for converting KanMX3 to URA3DepositorInsertURA3 (URA3 Budding Yeast)
UseYeast marker swapTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCR
Plasmid#60619PurposecrRNA expression plasmid on pRS423 backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yECitrine-Hygro
Plasmid#44646DepositorInsertyECitrine
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYC54
Plasmid#63909PurposeRecipient integrative vector to construct YFP tagged fusionsDepositorTypeEmpty backboneUseTagsYFPExpressionYeastMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T5
Plasmid#161492PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting T5 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pI4-MTU-DO-URA
Plasmid#160025PurposeIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Uracil selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-HIS
Plasmid#160005PurposeLow-copy expression vector for K. marxianus, histidine selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOP461
Plasmid#85934PurposeEncodes the Anti-Ebola 4G7 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.DepositorInsertAnti-Ebola 4G7 monoclonal antibody
UseTagsExpressionYeastMutationPromoterAOX1Available sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des13
Plasmid#110213PurposeYeast Surface Display of G6des13DepositorInsertG6des13
UseTagscmyc tagExpressionYeastMutationL A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95FPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Mup1-mScarlet2I
Plasmid#140152PurposePlasmid for pop-in/pop-out replacement of Mup1 with Mup1-mScarlet2IDepositorInsertMUP1
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTSK450
Plasmid#34552DepositorInsertMS2-CP-NLS
UseTagsmCherryExpressionYeastMutationPromoterMet25Available sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only