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pSNR52-sgTET

Plasmid

#46923

Purpose

Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE elements of pTET07 promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgRNA targeting endogenous TRE elements of pTET07 promoter

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pAG413GPD-ccdB-HA

Plasmid

#14238

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

3HA

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

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pDS2142

Plasmid

#184273

Purpose

Tet-off genome insertion system

Depositor

Dominique Sanglard

Article

Lemberg et al PLoS Pathog. 2022 Apr 11;18(4):e1010012.

Insert

CatTA

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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PSUC2-yEGFPCLN2PEST-pRS406

Plasmid

#64405

Purpose

SUC2 promoter driven GFP expression

Depositor

Claudia Vickers

Article

Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

Insert

SUC2 promoter (SUC2 Budding Yeast)

Use

Tags

yEGFPCLN2PEST (destabilized yeast EGFP)

Expression

Yeast

Mutation

Promoter

SUC2

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pR5-28

Plasmid

#22289

Depositor

Louise Prakash

Article

Pages et al Genetics. 2008 Sep . 180(1):73-82.

Insert

RAD5 (RAD5 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only
pXP322

Plasmid

#26839

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2010 Sep 10. ():.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pSP568

Plasmid

#139493

Purpose

Insertion of the pSTL1 promoter and 24 MS2 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pSTL1 24xMS2sl

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pSTL1

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pIAeBlue

Plasmid

#185891

Purpose

In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus

Depositor

Claudia Vickers

Insert

PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHES841

Plasmid

#89194

Purpose

Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Use

Tags

Expression

Yeast

Mutation

Promoter

pZ

Available since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDblet

Plasmid

#8848

Depositor

Joel Huberman

Article

Brun et al Gene. 1995 Oct 16. 164(1):173-7.

Insert

ARS doublet

Use

Yeast cloning vector

Tags

Expression

Yeast

Mutation

This vector contains, between AatII sites, a dire…

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pGM-ENO2-mCherry-YFP

Plasmid

#180570

Purpose

Dual fluorescent reporter for 5' UTR activity in yeast mCherry/YFP

Depositor

Joel McManus

Article

May et al Elife. 2023 May 25;12:e69611. doi: 10.75

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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GAL FLAG25Q+ProGFPp303

Plasmid

#15572

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q + Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region present

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pHM4 (U6 in pSE360)

Plasmid

#111281

Purpose

U6 snRNA coding sequence in pSE360(Ura) plasmid

Depositor

Christine Guthrie

Article

Madhani et al Genes Dev. 1990 Dec;4(12B):2264-77.

Insert

SNR6

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2796

Plasmid

#63641

Purpose

pCfB2796 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Jerome Maury, Irina Borodina

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

none

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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yHS1-M7A,H102A

Plasmid

#159152

Purpose

Cytosolic labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

yHS1-M7A,H102A

Use

Tags

Expression

Yeast

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

GPD

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pKT0139

Plasmid

#8731

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECitrine

Use

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with S65G, V68L, Q69M, S72A, T203Y

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET8

Plasmid

#107439

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET8 (MET8 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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LD211

Plasmid

#69183

Purpose

Integration of 2GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg11-2EGFP

Use

Tags

2xEGFP

Expression

Yeast

Mutation

Promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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LHP416-PAN1-HA

Plasmid

#32159

Depositor

Linda Hicke

Insert

PAN1 (PAN1 Budding Yeast)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pNS3

Plasmid

#131770

Purpose

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1

Use

Integration into δ-sites

Tags

Expression

Yeast

Mutation

E490G to make Cry2 into Cry2Olig

Promoter

PADH1

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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