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Showing: 761 - 780 of 1635 results

  3. pCS2+ cMyc-oDam_f_GFPoNLS

    Plasmid
    #85818
    Purpose
    iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    cMyc oDam_f_GFPoNLS
    Use
    Tags
    cMyc and oNLS (optimized nuclear localization sig…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    85818-plasmid-map-sequence-id-169572

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  4. pCS2+ oDam_f_GFPoNLS

    Plasmid
    #85819
    Purpose
    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    oDam_f_GFPoNLS
    Use
    Tags
    oNLS (optimized nuclear localization signal) C te…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    85819-plasmid-map-sequence-id-170478

    Enlarge

  5. pCS2+ cMyc Dam_f_GFPoNLS

    Plasmid
    #85820
    Purpose
    iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    cMyc Dam_f_GFPoNLS
    Use
    Tags
    cMyc and oNLS (optimized nuclear localization sig…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A.
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    85820-plasmid-map-sequence-id-170474

    Enlarge

  6. pNP148

    Plasmid
    #106358
    Purpose
    To insert a single copy of a construct at a site on Chr II, corresponding to a gain-of-function allele of gpa-12 expressed specifically in the adult epidermis.
    Depositor
    Jonathan Ewbank
    Insert
    col-62p_gpa-12gf_3'unc-54
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    106358-plasmid-map-sequence-id-204634

    Enlarge

  10. pNP160

    Plasmid
    #106362
    Purpose
    To insert a single copy of a construct at a site on Chr II, corresponding to RDE-1 expressed specifically in the intestine, for tissue-specific RNAi.
    Depositor
    Jonathan Ewbank
    Insert
    ttTi5605_SEC_mtl-2p_RDE-1_3'rde-1
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    106362-plasmid-map-sequence-id-204625

    Enlarge

  11. pNP172

    Plasmid
    #106363
    Purpose
    To insert a single copy of a construct at a site on Chr II, corresponding to mKate expressed specifically in the epidermis; can be used for N- or C-terminal fusion.
    Depositor
    Jonathan Ewbank
    Insert
    ttTi5605_SEC_col-12p_mkate2 x _3'tbb-2
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    106363-plasmid-map-sequence-id-211528

    Enlarge

  13. pSO7

    Plasmid
    #106365
    Purpose
    To insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the epidermis.
    Depositor
    Jonathan Ewbank
    Article
    Lee et al Virulence. 2018 Dec 31;9(1):648-658. doi
    Insert
    ttTi4348_ SEC _pcol-19_OspF_3'unc54UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    106365-plasmid-map-sequence-id-204930

    Enlarge

  14. pSO9

    Plasmid
    #106366
    Purpose
    To insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the intestine.
    Depositor
    Jonathan Ewbank
    Article
    Lee et al Virulence. 2018 Dec 31;9(1):648-658. doi
    Insert
    ttTi4348_ SEC _pvit-6_OspF_3'unc54
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    106366-plasmid-map-sequence-id-204924

    Enlarge

  19. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid
    #108362
    Purpose
    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans
    Depositor
    Luisa Cochella
    Article
    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461
    Inserts
    Ath-HEN1 (HEN1 Mustard Weed, Codon optimised for C. elegans)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    Use
    Tags
    2xFlag, MYC, T2A peptide, and TY1 linker
    Expression
    Worm
    Mutation
    Promoter
    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…
    Availability
    Academic Institutions and Nonprofits only
    108362-plasmid-map-sequence-id-209815

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Showing: 761 - 780 of 1635 results