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pDD364

Plasmid

#91831

Purpose

Lox511I-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Lox511I-SEC-Lox511I

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSR47

Plasmid

#69258

Purpose

pmyo-3::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Tags

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

myo-3

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAJ08

Plasmid

#14912

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis actin promoter

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pBCN42-R4R3

Plasmid

#34917

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

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pJH2069

Plasmid

#179224

Purpose

Pnmr-1::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-9-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

UNC-9 (unc-9 Nematode)

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pnmr-1

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pJRK138

Plasmid

#173746

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CRY2(olig)::VHH(GFP)::unc-54 3' UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Prps-0

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1359 - Intron GG2 - smu-1

Plasmid

#159805

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - smu-1

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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L3670

Plasmid

#1586

Depositor

Andrew Fire

Type

Empty backbone

Use

Tags

let858Tsa, ATG-MitLS, gfpN, IVSR, gfpS65C, IVSS, …

Expression

Worm

Mutation

Promoter

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

Plasmid

#164266

Purpose

sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6 promoter from W05B2.8

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1850

Plasmid

#154328

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

ttTi5605 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Tags

Expression

Worm

Mutation

F+E mutations in sgRNA

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1849

Plasmid

#154327

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

ttTi4348 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Tags

Expression

Worm

Mutation

F+E mutations in sgRNA

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMLS714

Plasmid

#188886

Purpose

MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS719

Plasmid

#188894

Purpose

miniMos Arm sgRNA expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

PU6(K09B11.12)::miniMos Arm sgRNA

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM232 - [Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR]

Plasmid

#159898

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAV1951

Plasmid

#37832

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Pnhx-2mCherry

Use

Tags

Expression

Worm

Mutation

Promoter

nhx-2

Available since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only
pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159867

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSM12

Plasmid

#139035

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

dendra2 (no STOP codon)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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L4404

Plasmid

#1648

Depositor

Andrew Fire

Type

Empty backbone

Use

RNAi

Tags

T7p, lacZN, OriF1>>, OriF1<<, T3p

Expression

Worm

Mutation

Promoter

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pDD382

Plasmid

#91830

Purpose

Lox2272-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Lox2272-SEC-Lox2272

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pGC76

Plasmid

#19641

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<4xNLS-GFP::let-858(3’)

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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