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Plasmid#191053Purposelim-6 fluorescent neural reporter driving nuclear mNeptune2.5 and CyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertlim-6 (lim-6 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY45
Plasmid#191049Purposegcy-35 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertgcy-35 (gcy-35 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS1-his-24::tagBFP2
Plasmid#124321PurposeExpressing Nuclear Localized tagBFP2DepositorInserttagBFP2 (his-24 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWZ194
Plasmid#163640Purposerps-27>DHB::2xmKate2-P2A-H2B::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome IDepositorInsertDHB::2mKate2-P2A-H2B::GFP::3xHA (his-58 Synthetic, Nematode)
UseCRISPRTags2xmKate2 and GFPExpressionWormMutationPromoterrps-27Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH4504
Plasmid#191702PurposePnmr-1 long::TeTx::wCherry unc-54 3' UTR C.elegans AVA/other neurons expression of TeTx RFPDepositorInsertTetanus Toxin Light Chain
UseTagswCherryExpressionWormMutationPromoterPnmr-1 longAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH1924
Plasmid#179205PurposePnmr-1::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-7-wCherryDepositorInsertUNC-7
UseTagswCherryExpressionWormMutationPromoterPnmr-1Available sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2
Plasmid#182359PurposePositive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.DepositorInsertUltra-pan-neuronal positive control pUNP::gfp(NLS)
UseTagsExpressionWormMutationPromoterPromoter Ultra Pan NeuronalAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pSAH49 - [PVQp | mScarlet | tbb-2 UTR]
Plasmid#200325PurposemScarlet BioPart for PVQ neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[PVQp | wrmscarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003755, nlp-17Available sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH3456
Plasmid#179581PurposePnmr-1::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 BFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagsBFPExpressionWormMutationPromoterPnmr-1Available sinceMarch 2, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
lis-1::L4440
Plasmid#11336DepositorInsertlis-1 (lis-1 Nematode)
UseRNAiTagsExpressionWormMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pKK1 -pFlp-18::mito4x-GCaMP6f
Plasmid#194969PurposeFluorescent reporter of mitochondrial matrix Ca2+.DepositorInsert4 repeats of Cox8 presequence plus GCaMP6f
UseTagsExpressionWormMutationPromoterFlp-18Available sinceJune 21, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBCN22-R4R3
Plasmid#26302DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSAH71 - [AWAp | wrmScarlet | tbb-2 UTR]
Plasmid#200345PurposemScarlet BioPart for AWA neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[AWAp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003856, odr-10Available sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH72 - [AWAp | gfp | tbb-2 UTR]
Plasmid#200346Purposegfp BioPart for AWA neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[AWAp | gfp | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003856, odr-10Available sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-YFP-Smo
Plasmid#31618DepositorInsertSmo (Smo Mouse)
UseZebrafish, avian expressionTagsYFPExpressionMammalian and WormMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMB62
Plasmid#47944PurposeExpresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, egl-13 NLS, and mEGFPExpressionWormMutationPromotereef-1A.1 (eft-3)Available sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159858PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM270 - MCS mosTI hygroR target
Plasmid#159826PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertMCS mosTI hygroR target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH4531
Plasmid#132366PurposePrgef-1 DAF-2 unc-54 3' UTR C.elegans pan-neural expression of DAF-2. This plasmid has replaced plasmid pJH664 from the Zhen Lab.DepositorInsertDAF-2 (daf-2 Nematode)
UseTagsnoneExpressionWormMutationPromoterPrgef-1Available sinceSept. 22, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits