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pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159850

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJK2020

Plasmid

#171932

Purpose

express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon

Depositor

Judith Kimble

Article

Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.

Insert

Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR

Use

Tags

superfolder GFP, mCherry

Expression

Worm

Mutation

Promoter

-

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2

Plasmid

#182353

Purpose

GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJH4504

Plasmid

#191702

Purpose

Pnmr-1 long::TeTx::wCherry unc-54 3' UTR C.elegans AVA/other neurons expression of TeTx RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Tetanus Toxin Light Chain

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pnmr-1 long

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pJH1924

Plasmid

#179205

Purpose

Pnmr-1::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-7-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

UNC-7

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pnmr-1

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Plasmid

#159813

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMS051

Plasmid

#137025

Purpose

SapTrap mScarlet-I-GLO donor

Depositor

Daniel Dickinson, Bob Goldstein

Insert

C. elegans GLO-optimized mScarlet

Use

CRISPR; Saptrap donor for cloning repair templat…

Tags

C. elegans GLO-optimized mScarlet

Expression

Worm

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGLOW79

Plasmid

#177339

Purpose

C. elegans mNeonGreen co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-3::mNeonGreen

Use

Tags

mNeonGreen

Expression

Worm

Mutation

Promoter

myo-3

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSM_ser-2prom3p::myr-mCherry

Plasmid

#164223

Purpose

Expression of membrane-localized mCherry driven by the ser-2promo3 promoter. For use as a PVD neuron marker in C. elegans.

Depositor

Yan Zou

Article

Feng et al Cell Rep. 2020 Sep 8;32(10):108101. doi:

Insert

myr-mCherry

Use

Tags

Expression

Worm

Mutation

Promoter

ser-2promo3

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ178 - cxTi10882_MCS

Plasmid

#19331

Depositor

Erik Jorgensen

Article

Christian et al

Insert

cxTi10882 targetting region

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his-24::CyOFP

Plasmid

#124347

Purpose

Nuclear Localized Pan-Neuronal CyOFP

Depositor

Andrew Leifer

Insert

rab-3, CyOFP (rab-3 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)

Plasmid

#193049

Purpose

Encodes G-418 resistance

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

prsp-27::NeoR::unc-54 3’ UTR

Use

Tags

Expression

Worm

Mutation

Promoter

prsp-27

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSH1

Plasmid

#90295

Purpose

Expresses the fat-7 gene in C. elegans intestine

Depositor

Anne Brunet

Article

Han et al Nature. 2017 Apr 13;544(7649):185-190. d

Insert

fat-7 (fat-7 Nematode)

Use

Tags

No

Expression

Worm

Mutation

Promoter

ges-1

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pJH3456

Plasmid

#179581

Purpose

Pnmr-1::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 BFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Use

Tags

BFP

Expression

Worm

Mutation

Promoter

Pnmr-1

Available since

March 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJW1582

Plasmid

#154312

Purpose

mNeonGreen^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mNeonGreen^SEC^AID*::3xFlag

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM253 - sgRNA mosTI hygroR

Plasmid

#159827

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI hygroR

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMLS262

Plasmid

#73718

Purpose

C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Psnt-1::2xNLS-FLP-D5

Use

Tags

Expression

Worm

Mutation

G5D

Promoter

Psnt-1

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pJH2523

Plasmid

#191358

Purpose

Punc-25-GCaMP3::UrSL2 ::wCherry unc-54 3' UTR C.elegans DD neurou expression of GCaMP3 RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GCaMP3

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Punc-25

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAS1-odr-4P::his-24::mNeptune

Plasmid

#124342

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

odr-4, mNeptune (odr-4 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pPD49_78

Plasmid

#1447

Depositor

Andrew Fire

Type

Empty backbone

Use

Tags

hsp16-415', hsp16-25', IVSA, rf2128

Expression

Worm

Mutation

Promoter

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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