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Plasmid#91167PurposeCas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:barDepositorInsertAtCas9_D10A+H840A (dead)
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pNOS::cogGFP (C132, JBEI-15898)
Plasmid#110145PurposeTransformation and expression of Csy4 and cogGFP proteins in plantsDepositorInsertCsy4 (ATCS Mustard Weed)
UseTagscogGFPExpressionBacterial and PlantMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVL45 - pPXY>>
Plasmid#116011Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpPXY:GR-LhG4:tPXY:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH68
Plasmid#128188PurposeLevel1 ZmUBIp::SpCas9-NG (human codon optimized):NOStDepositorInsertZmUBIp::SpCas9-NG (human codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH100
Plasmid#128205Purposeimproved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)DepositorInsertimproved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTC151
Plasmid#70015PurposeTALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a non-replicating, mutant version of the Gemini Viral RepliconDepositorInsertNuclease (TALENs) + Donor + GVR
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFH82
Plasmid#128193PurposeLevel1 ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOStDepositorInsertZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH93
Plasmid#128196PurposeLevel1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOStDepositorInsertZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH101
Plasmid#128206PurposesgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)DepositorInsertsgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH59
Plasmid#128185PurposeLevel1 ZmUBIp::StCas9 (Arabidopsis codon optimized):NOStDepositorInsertZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH81
Plasmid#128192PurposeLevel1 ZmUBIp::ScCas9 (wheat codon optimized):NOStDepositorInsertZmUBIp::ScCas9 (wheat codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB133
Plasmid#68587PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJG484
Plasmid#91194PurposeWDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor)DepositorInsertnTaCas9_H840A+gUbi8+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM860
Plasmid#84179Purposetarget miRNA860 for destruction in Arabidopsis thalianaDepositorInsertSTTM860
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM1887
Plasmid#84105Purposetarget miRNA1887 for destruction in Arabidopsis thalianaDepositorInsertSTTM1887
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM447
Plasmid#84138Purposetarget miRNA447 for destruction in Arabidopsis thalianaDepositorInsertSTTM447
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM163
Plasmid#84089Purposetarget miRNA163 for destruction in Arabidopsis thalianaDepositorInsertSTTM163
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
5aa SunTag VP64 AP3
Plasmid#115484PurposeCRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PABE-3
Plasmid#115624PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertwtTadA-TadA7.10-NLS-nCas9-NLS
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
PABE-6
Plasmid#115627PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertwtTadA-TadA7.10-nCas9-2*NLS
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only