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pZA68

Plasmid

#174781

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V-eGFP, Kmr plant selection marker

Use

Tags

eGFP

Expression

Plant

Mutation

L17E/D481V

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pZA66

Plasmid

#174779

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V-eGFP, Kmr plant selection marker

Use

Tags

eGFP

Expression

Plant

Mutation

D481V

Promoter

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZA65

Plasmid

#174778

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-eGFP, Kmr plant selection marker

Use

Tags

eGFP

Expression

Plant

Mutation

WT

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA67

Plasmid

#174780

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E-eGFP, Kmr plant selection marker

Use

Tags

eGFP

Expression

Plant

Mutation

L17E

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)

Plasmid

#160635

Purpose

TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

Insert

AcrIIA4

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRATIO2251

Plasmid

#141305

Purpose

Gateway cloning compatible ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

Firefly Luciferase or LUC2 and mScarlet-I

Expression

Plant

Mutation

Promoter

UBQ10 promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB106-OEP7(1–50)

Plasmid

#100602

Purpose

Binary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plants

Depositor

Yutaka Kodama

Article

Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

Insert

OEP7(1–50)-Citrine (OEP7 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDGB3_Omega1_Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos (GB1677)

Plasmid

#160641

Purpose

Module for estradiol-inducible exp of the PhiC31 integrase gene. Includes TU for constitutive exp of an estradiol-inducible transcription activator and TU for estradiol-inducible exp of PhiC31.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

ERLexABDGal4AD / PhiC31

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pG20_LUC2_Hyg

Plasmid

#159707

Purpose

pGREEN-based cloning construct for expression in planta

Depositor

Hans-Henning Kunz

Article

Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

Type

Empty backbone

Use

Tags

luciferase

Expression

Plant

Mutation

Promoter

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pG20_GUS_Blp

Plasmid

#159706

Purpose

pGREEN-based cloning construct for expression in planta

Depositor

Hans-Henning Kunz

Article

Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

Type

Empty backbone

Use

Tags

GUS

Expression

Plant

Mutation

Promoter

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pEE386

Plasmid

#149284

Purpose

T-DNA encoding TRV2 with gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1:tNos

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEE388

Plasmid

#149286

Purpose

T-DNA encoding TRV2 with mFT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1_mFT:tNos

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only
pEE385

Plasmid

#149289

Purpose

T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA2_TrunFT:tNos

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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MIGS5.2 (FF576)

Plasmid

#35254

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pMpGWB216

Plasmid

#68607

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Nuclear localization signal-tagged tdTomato (tdTo…

Expression

Plant

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB229

Plasmid

#68620

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

tdTomato

Expression

Plant

Mutation

Promoter

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAtCas9-dead_1

Plasmid

#91165

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A+H840A (dead)

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, H840A

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pG20_GUS_Hyg

Plasmid

#159705

Purpose

pGREEN-based cloning construct for expression in planta

Depositor

Hans-Henning Kunz

Article

Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

Type

Empty backbone

Use

Tags

GUS

Expression

Plant

Mutation

Promoter

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGREAT4

Plasmid

#170892

Purpose

GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Tags

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

pG10-90

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM162

Plasmid

#84088

Purpose

target miRNA162 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM162

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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