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Plasmid#130835PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertSUN2
UseTagsmCherry-FLAG-HAExpressionPlantMutationDeletion of SUN domainPromoterCaMV 35SAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0058
Plasmid#117539PurposeLevel1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamianaDepositorInsertsgRNA (SaCas9)
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
lvl0 TUB9t
Plasmid#153385PurposePlant terminatorDepositorInsertTUB9 terminator
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
lvl0 35St
Plasmid#153382PurposeCauliflower mosaic virus terminator for plant expressionDepositorInsert35S terminator
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0038
Plasmid#117605PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029)DepositorInsert[35S:LbCas12a(pEPOR1CB0013) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-SEIPIN3-Ndelta
Plasmid#96981PurposeExpress N-terminal truncated Arabidopsis SEIPIN3 gene in plants.DepositorInsertSEIPIN3 (AT2G34380 Mustard Weed)
UseTags6xHis and GFPExpressionPlantMutationA segment of 507 nucleotides are deleted from the…Promoter35SAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB423
Plasmid#68688PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTriple repeats of Citrine (3xCitrine)ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
35S-frGFP-nosT
Plasmid#80128PurposeTransient expression vector for frGFP in plantsDepositorTypeEmpty backboneUseTagsfrGFPExpressionPlantMutationPromoterCaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTX208
Plasmid#89268PurposeExpress STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01 and OsYSA-gRNA02DepositorInsertOsYSA-gRNA01 and OsYSA-gRNA02
UseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterMaize Ubiquitin1 promoterAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd SUP
Plasmid#115489PurposeCRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC50
Plasmid#104824PurposeCRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.DepositorInsertGlyma.08g081600, Glyma.05g126600
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJG493
Plasmid#91197PurposeWDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor)DepositorInsertgUbi1+gUbi8+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMU103
Plasmid#73934PurposeThis is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.DepositorInsertbar and GUS cassettes
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEELA
Plasmid#79752Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter2xPro35SAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
5aa SunTag VP64 CLV3
Plasmid#115485PurposeCRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJIT163-AFID-3
Plasmid#167359PurposeFor inducing predictable deletions in protoplasts and wheat plantsDepositorInsertA3A-Cas9-UDG-T2A-AP lyase
UseTagsExpressionPlantMutationWTPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
PABE-7-NG
Plasmid#138142PurposeTargeted A to G in rice protoplastsDepositorInsertwtTadA-TadA7.10-nCas9-NG-3XNLS
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 35s:PIF6:tNos (GB0170)
Plasmid#160606PurposeTU for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switchDepositorInsertPIF6
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)4
Plasmid#127522PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 4 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 4 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_26K
Plasmid#91152PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TALEN, Plant Selection: PvUbi2:barDepositorInsertEngineering Reagent: ZmUbi:TALEN
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only