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Plasmid#91136PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSS02:cyt-Peredox-mCherry
Plasmid#161743Purposecytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plantsDepositorInsertcyt-Peredox-mCherry
UseTagsExpressionPlantMutationPromoterUbiquitin 10Available sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLXMA L2A
Plasmid#153360PurposeExpression vector for plants with medium-copy number origin of replicationDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
PHP665
Plasmid#132352PurposeC1 over-expression cassette for Zea maysDepositorInsertC1
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHP11004
Plasmid#132353PurposeR over-expression cassette for Zea maysDepositorInsertR
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AL13Rb-HsfA2
Plasmid#161012PurposePP7-tagged reporter driven by the Arabidopsis HsfA2 promoterDepositorInsertPP7 and H2B-mScarlet
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
AL12R-EF-Tu
Plasmid#161013PurposePP7-tagged reporter driven by the Arabidopsis EF-Tu promoterDepositorInsertPP7 and H2B-mScarlet
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
HSP70-pp7i-mCh-UPG
Plasmid#161011PurposeC-terminal mCherry fusion to Arabidopsis HSP70 with intronic PP7DepositorInsertPP7 and H2B-mScarlet
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGREAT17
Plasmid#170905PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
BCJJ340
Plasmid#117502PurposeUBI10:Cas9:E9 Golden Gate Level 1 Position 2DepositorInsertBpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
UseCRISPRTagsExpressionPlantMutationPromoterAtUBI10Available sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFH52
Plasmid#128181PurposeLevel1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOStDepositorInsert2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
lvl0 UBQ5t
Plasmid#153383PurposePlant terminatorDepositorInsertUBQ5 terminator
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRATIO2151
Plasmid#141300PurposeGateway cloning compatible ratiometric binary vectorDepositorTypeEmpty backboneUseTagsFirefly Luciferase or LUC2 and mScarlet-IExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBGC155-MXMT
Plasmid#79818PurposeGFP(S65T)-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsGC155/GFP (155–239)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBGN155
Plasmid#74992PurposeGFP(S65T)-based BiFC vector for transient expression of sGN (1-155) in plantsDepositorTypeEmpty backboneUseReporter plasmidTagsGFP (1–155)/GN155ExpressionPlantMutationPromoterCaMV35SAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)
Plasmid#160607PurposeTU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.DepositorInsertCUP2Gal4AD
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB436
Plasmid#68701PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21D
Plasmid#91131PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFH85
Plasmid#128198Purposeclassic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)DepositorInsertclassic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDB1alpha11:empty
Plasmid#106207PurposeGoldenBraid level 1 Alpha plant expression vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only