-
Plasmid#149291PurposeT-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2DepositorInsertp35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUPD2:35S-TMV-Rep-MP
Plasmid#106217PurposeProvides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp polymerase with intron, movement protein and sgCP promoter) as level 0 GoldenBraid part (GGAG-AATG)DepositorInsertInfectious clone of Tobacco mosaic virus, 5' part
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB105-MpTubulin
Plasmid#100588PurposeBinary vector for expression of Citrine-fused MpTubulin beta 3 (Citrine-MpTubulin) to visualize microtubules in plantsDepositorInsertCitrine-MpTubulin
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMP-APr
Plasmid#61184PurposeFluorescent reporter for apoplast MtBCP1sp-mCherry expressed under MtPT4 promoterDepositorInsertMtBCPsp-mCherry
UseTagsmCherryExpressionPlantMutationPromoterMtPT4Available sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ289-RVR
Plasmid#138127PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoterDepositorInsertErCas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD513R, K519V and N523RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
TF101-STTM-miR156/157
Plasmid#84811PurposeTurning off/down miR156/157 in SoybeanDepositorInsertSTTM-miR156/157
UseTagsExpressionPlantMutationPromoterunknownAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_10A
Plasmid#91207PurposeDirect Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: noneDepositorInsert35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-T679A-NES
Plasmid#118940PurposeUsed to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pARS3_MUbCAS9_GW
Plasmid#128646PurposeT-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNADepositorTypeEmpty backboneUseTagsHAExpressionPlantMutationPromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXSE901B
Plasmid#91713PurposeCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistanceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTX168
Plasmid#89257PurposeExpress STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZYIntExc
Plasmid#139673PurposeExpression cassette for phiC31 Excisionase in T-DNA vectorDepositorInsertphiC31 Excisionase
UseTagsExpressionPlantMutationPromoterMaize UbiquitinAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDest_pK7WG2_pRPS5A_CTP OleGFP
Plasmid#171723PurposeptpTALECD vector assembly, Destination vector;DepositorInsertRPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2
UseTagsExpressionPlantMutationPromoterAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141C2.0
Plasmid#99905PurposeExpress single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE-NG
Plasmid#138141PurposeTargeted C-to-T in rice protoplastsDepositorInsertAPOBEC3A-nCas9-NG-UGI-NLS
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH66
Plasmid#131765PurposeLevel1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOStDepositorInsertZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH7m34GW_RcCHAD-mCherry
Plasmid#127784PurposeRcCHAD-mCherry expression in plantsDepositorInsertUbi10p:RcCHAD-mCherry
UseTagsmCherryExpressionPlantMutationPromoterUbiquitin10 promoter + 5' UTRAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:dCas9:Tnos (GB1191)
Plasmid#68223PurposeTranscriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoterDepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removed; human codon optimis…Promoter35SAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINT4
Plasmid#127530PurposePlasmid encodes A. thaliana codon optimized Integrase 4.DepositorInsertIntegrase 4 coding sequence codon optimized for A. thaliana expression.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB314
Plasmid#68642PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only