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Plasmid#91206PurposeTransformation, Type: non-T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only
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tRNA-gRNA position [D1_n-1] (GB1208)
Plasmid#75409PurposetRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)DepositorInserttRNA-gRNA position [D1_n-1]
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRATIO2112
Plasmid#141296PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmScarlet-I and mVenusExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB119
Plasmid#68573PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ269D
Plasmid#164717PurposeGateway entry clone (attL1 & attR5) PmCDA1-CBE_V04 for C-T base editingDepositorInsertzCas9(D10A)-PmCDA1-UGI-T2A-MS2-UGI)
UseCRISPR; Gateway compatible pmcda1-cbe_v04 entry c…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM62624
Plasmid#169094PurposeLow copy MoClo level 2 binary vector for plant transformationDepositorTypeEmpty backboneUseMoclo cloning vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:(MCS)-S11
Plasmid#108179PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEE495
Plasmid#149291PurposeT-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2DepositorInsertp35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTX168
Plasmid#89257PurposeExpress STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB3omega1_KanR_BastaR
Plasmid#186426Purposeoptimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)DepositorInsertsKanR
BastaR
UseSynthetic Biology; Binary vector for escherichia …TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterCaMV 35S and PnosAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha11_SulR
Plasmid#186424Purposetranscriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assemblyDepositorInsertSulR
UseSynthetic Biology; Binary vector for escherichia …TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM173
Plasmid#84101Purposetarget miRNA173 for destruction in Arabidopsis thalianaDepositorInsertSTTM173
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pARS3_MUbCAS9_GW
Plasmid#128646PurposeT-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNADepositorTypeEmpty backboneUseTagsHAExpressionPlantMutationPromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-T679A-NES
Plasmid#118940PurposeUsed to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMP-APr
Plasmid#61184PurposeFluorescent reporter for apoplast MtBCP1sp-mCherry expressed under MtPT4 promoterDepositorInsertMtBCPsp-mCherry
UseTagsmCherryExpressionPlantMutationPromoterMtPT4Available sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
LLP762_L2pV1_OR-OCS_TCTP-Flp_s35S-Cre
Plasmid#192421PurposeTo act as the 2-input state of the OR gate.DepositorInsertAct2::FRT-loxP-OCS-FRT-loxP::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_232
Plasmid#91121PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: ToLCVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141C2.0
Plasmid#99905PurposeExpress single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD2:35S-TMV-Rep-MP
Plasmid#106217PurposeProvides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp polymerase with intron, movement protein and sgCP promoter) as level 0 GoldenBraid part (GGAG-AATG)DepositorInsertInfectious clone of Tobacco mosaic virus, 5' part
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH7m34GW_RcCHAD-mCherry
Plasmid#127784PurposeRcCHAD-mCherry expression in plantsDepositorInsertUbi10p:RcCHAD-mCherry
UseTagsmCherryExpressionPlantMutationPromoterUbiquitin10 promoter + 5' UTRAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only