Addgene

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pORFE0005

Plasmid

#112073

Purpose

AtDeadCAS9 D10A H840A without stop codon module CDS1ns

Depositor

Christian Chevalier

Insert

AtDeadCAS9 D10A H840A without stop codon

Use

CRISPR

Tags

Expression

Plant

Mutation

Plant codon optimized dCas9 D10A S. pyogenes

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM159

Plasmid

#84084

Purpose

target miRNA159 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM159

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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lvl0 mTFP1 CTAG

Plasmid

#153400

Purpose

Cyan fluorescent protein with FLAG tag

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

mTFP1 CTAG

Use

Tags

FLAG tag

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFH34

Plasmid

#126008

Purpose

Level0 Arabidopsis AtU6-26 Pol III promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Arabidopsis AtU6-26 Pol III promoter

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH45

Plasmid

#125810

Purpose

Level0 nSpCas9-ABE7.10 base changer, human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

nSpCas9-ABE7.10 base changer, human codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAGM7547

Plasmid

#169091

Purpose

MoClo level 1 35S promoter-Db5GT-35S terminator transcription unit

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-Db5GT-35S terminator

Use

Moclo cloning vector

Tags

Expression

Plant

Mutation

Promoter

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM7523

Plasmid

#169090

Purpose

MoClo level 1 35S promoter-BvDODA1-Ocs terminator transcription unit

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-BvDODA1-Ocs terminator

Use

Moclo cloning vector

Tags

Expression

Plant

Mutation

Promoter

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM7535

Plasmid

#169089

Purpose

MoClo level 1 35S promoter-BvCYP76AD1-Nos terminator transcription unit

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-BvCYP76AD1-Nos terminator

Use

Moclo cloning vector

Tags

Expression

Plant

Mutation

Promoter

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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L1_GATA23_2

Plasmid

#195912

Purpose

Level 1 PhiC31 cassette with LR dvp promoter: pGATA23-PhiC31-NLS-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pGATA23-PhiC31-NLS-tUBQ1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB528

Plasmid

#74870

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB308

Plasmid

#68636

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

MpEF1alpha promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pFH57

Plasmid

#125883

Purpose

Level0 Soybean GmUBI Pol II promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Soybean GmUbi Pol II promoter

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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UBC1cer60G

Plasmid

#161016

Purpose

ubiquitously weakly expressed 60-meric GFP nanocage

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

60meric GFP nanocage

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pGREAT18

Plasmid

#170906

Purpose

GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Tags

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

pAtUBQ10

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pRW006-SM-destination-pro35S-Cas9

Plasmid

#104439

Purpose

Destination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial and Plant

Mutation

Promoter

35S

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-35S-GW-Luc

Plasmid

#80678

Purpose

binary DEST vector for plant expression

Depositor

Imre Somssich, Nico Dissmeyer

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Pro35S

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pUBN-paGFP-Dest

Plasmid

#105108

Purpose

fluorescent tagging in stably transformed plants

Depositor

Christopher Grefen

Article

Blatt et al Methods Mol Biol. 2014;1062:487-507. doi

Type

Empty backbone

Use

Tags

paGFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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UBC1cer120G

Plasmid

#161017

Purpose

ubiquitously weakly expressed 120-meric GFP nanocage

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

120meric GFP nanocage

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE652

Plasmid

#153230

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pXEE401BG

Plasmid

#91717

Purpose

CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance

Depositor

Qi-Jun Chen

Article

Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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