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pRGEB-VirD2-Cas9

Plasmid

#139803

Purpose

Expressing VirD-Cas9 fusion under ubiquitin promoter

Depositor

Magdy Mahfouz

Article

Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

Insert

VirD2-Cas9

Use

Tags

VirD2-Cas9

Expression

Plant

Mutation

Promoter

Ubiquitin

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL86955OD

Plasmid

#86179

Purpose

GG compatible single transcript acceptor BASTA

Depositor

Mark Youles

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pG20_MCS_blp

Plasmid

#159710

Purpose

pGREEN-based cloning construct for expression in planta

Depositor

Hans-Henning Kunz

Article

Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pG20_MCS_Hyg

Plasmid

#159709

Purpose

pGREEN-based cloning construct for expression in planta

Depositor

Hans-Henning Kunz

Article

Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB313

Plasmid

#68641

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Mutation

Promoter

MpEF1alpha promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pRATIO1212

Plasmid

#141299

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

mScarlet-I and mVenus

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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DCIP

Plasmid

#193860

Purpose

Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

NLS-mCherry-GFP1-10

Use

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

35S

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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L1_ARF19_4

Plasmid

#195916

Purpose

Level 1 PhiC31 cassette with LR dvp promoter: pARF19-PhiC31-DST-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pARF19-PhiC31-DST-tUBQ1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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CDS12_mTurquoise2

Plasmid

#136089

Purpose

FP for N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mTurquoise2(noStop)

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pdCas9-PBE

Plasmid

#98161

Purpose

Expresses CRISPR base editor in protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-dCas9-UGI-NLS

Use

Tags

Expression

Plant

Mutation

Promoter

Ubi

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMAP L2B

Plasmid

#153357

Purpose

Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 UBQ10p

Plasmid

#153363

Purpose

Plant promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

UBQ10 promoter

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB401

Plasmid

#68666

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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CDS_LUC

Plasmid

#136083

Purpose

Firefly luciferase

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_LUC

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAK-EL-01

Plasmid

#125760

Purpose

Endlinker for tRNA-gRNA system when using only one guide

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK-EL-03

Plasmid

#125762

Purpose

Endlinker for tRNA-gRNA system when using only three guides

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK-EL-04

Plasmid

#125763

Purpose

Endlinker for tRNA-gRNA system when using only four guides

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK-EL-05

Plasmid

#125764

Purpose

Endlinker for tRNA-gRNA system when using only five guides

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ-dzCas9-NG-Act3.0

Plasmid

#158415

Purpose

CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMC-1-35S_Long-6

Plasmid

#197698

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

35S promoter (1.3kb)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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