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Plasmid#89261PurposeExpress STU (Single Transcriptional Unit) Cas9 nickase with Maize Ubiquitin1 promoter in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only
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pGEL066
Plasmid#124897PurposeFor genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editorDepositorTypeEmpty backboneUseCRISPRTags3xFlagExpressionPlantMutationPromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB421
Plasmid#74815PurposeGateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RV
Plasmid#188544PurposeGateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editingDepositorInsertLbCas12a-RV
UseCRISPR; Gateway compatible lbcas12a-rv entry cloneTagsNLSExpressionPlantMutationG146R, R182VPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZA31
Plasmid#158509PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc
UseTags4xMycExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:(MCS)-S11-DI-GFP1–9
Plasmid#108260PurposeBinary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB524
Plasmid#74866PurposeGateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGD283
Plasmid#129733PurposeDestination plasmid containing the 35S:XVE estradiol inducible driver and the A-G overhangs for GreenGate cloning of an expression casette for estradiole-inducible expression in plants.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
UPG
Plasmid#161003Purposeubiquitous expression of PCP-GFPDepositorInsertPCP-GFP
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Golgi
Plasmid#183169PurposeConstruct for transient expression of Golgi apparatus-targeted mCherry in plants.DepositorInsertmCherry-Golgi
UseTagsAtRER1B, A. thaliana endoplasmic reticulum retrie…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdCas9 (GB1079)
Plasmid#75399PurposeProvides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusionsDepositorInsertCas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB529
Plasmid#74871PurposeGateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTF-FLPe
Plasmid#139672PurposeExpression cassette for FLPe recombinase in T-DNA vectorDepositorInsertFLPe recombinase
UseTagsExpressionPlantMutationPromoterMaize UbiquitinAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAP L1A
Plasmid#153343PurposeMobius Assembly cloning and expression vector for plants with high-copy number origin of replicationDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM37443
Plasmid#153218PurposeLevel 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB701NL3F10H
Plasmid#141288PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)DepositorTypeEmpty backboneUseLuciferase; GatewayTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMU-TLEr
Plasmid#61187PurposeFluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under AtUBQ10 promoterDepositorInsertMtRAB7A2
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRETvr-2in1-CC
Plasmid#105117PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmVenus/tagRFPExpressionPlantMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10
Plasmid#197966PurposeT-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.DepositorInsertspco-vCasphi-2
AtPDS3 gRNA10
UseCRISPRTagsExpressionPlantMutationPromoterCmYLCVp and pUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pbZIP9:GFP-GUS
Plasmid#172483PurposeGFP-GUS driven by the bZIP9 promoterDepositorInsertbZIP9 promoter (BZIP9 Mustard Weed)
UseTagsGFP and GUSExpressionPlantMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only