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Plasmid#161014PurposePP7-tagged reporter driven by the Arabidopsis HSP101 promoterDepositorInsertPP7 and H2B-mScarlet
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-iSpyMac
Plasmid#149553PurposeGateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMsDepositorInsertiSpyMacCas9
UseCRISPR; Gateway compatible zispymaccas9 entry clo…Tags3X FLAG, NLS and NLSExpressionPlantMutationR221K, N394K, Protospacer interacting domain form…PromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDESTpK7WG-RPS5Ap-mito
Plasmid#163145PurposePlant mitoTALEN vector assembly, Destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the RPS5A promoterDepositorInsertRPS5A promoter and mitochondrial presequence in front of attR1 in pk7WG
UseTagsExpressionPlantMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ145-ZmUbi-tRNA
Plasmid#158403PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRATIO1112
Plasmid#141066PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmScarlet-I and mVenusExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132A2.0
Plasmid#99887PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9_3
Plasmid#91158PurposeCas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:barDepositorInsertAtCas9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFH113
Plasmid#128210Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNC-Cam2304-35S
Plasmid#193385PurposeNimble cloning-compatible vector for plant ectopic expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2-U6
Plasmid#173157PurposeSingle guide RNA cassette under U6 promoterDepositorInsertsgRNA cassette under U6 promoter
UseTagsExpressionPlantMutationPromoterAtU6-26Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFGC-I2Cas9
Plasmid#173158PurposeExpresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.DepositorInsertshSpCas9
pNAP:dsRED:tNOS
pMAS:BAR:tMAS
ICU2 upstream regulatory region
UseTags3xFLAG-NLS and NLSExpressionPlantMutationContains a D169N mutation with no visible effect …PromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTT135
Plasmid#207924PurposePremade UBI10 driven expression of LhG4 (pUBQ10:GR-LhG4:tHSP18.2M) for braidingDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMP108
Plasmid#207928PurposePremade plasma membrane marker, pUBQ10:PiP1;4:PmScarlet-I:tHSP18.2M:FastRed, for braidingDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBD001
Plasmid#201330PurposepDefence14:Eluc (defence-induced promoter with green-shifted luciferase expression cassette)DepositorInsertpDefence14:Eluc
UseTagsExpressionPlantMutationremoval of BsaI and BpiI sitesPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ165
Plasmid#109327PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterPromoter EC1.2 enhancer fused to EC1.1 promoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
lvl0 FAD2t
Plasmid#153377PurposePlant terminatorDepositorInsertFAD2 terminator
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ143-ZmUbi
Plasmid#138107PurposeGolden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by Zea mays Ubi promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35s:dCas:BRD:tNos (GB1172)
Plasmid#75401PurposeTranscriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional RepressorDepositorInsertdCas9:BRD
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
PEP103E
Plasmid#97389Purposebinary construct of plastid-targeted sfGFP1-10 for plant expressionDepositorInsertsfGFP1-10OPT
UseTagsPlastid targetExpressionPlantMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEP105E
Plasmid#97391Purposebinary construct of peroxisome-targeted sfGFP1-10 for plant expressionDepositorInsertsfGFP1-10OPT
UseTagsPeroxisome targetExpressionPlantMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only