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P527

Plasmid

#139741

Purpose

Individual component of Caffeic Acid biosynthesis pathway, RcTAL

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

TAL

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-vCASphi_E9t_MCS_version2

Plasmid

#197961

Purpose

T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pco-vCasphi-2

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

pUBQ10

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-nCASphi_E9t_MCS_version2

Plasmid

#197962

Purpose

T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pco-nCasphi-2

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

pUBQ10

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-AtUbi10-6

Plasmid

#197700

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AtUbi10 promoter

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWB327

Plasmid

#68655

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

TagRFP

Expression

Plant

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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PEP102E

Plasmid

#97388

Purpose

binary construct of nuclear-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Use

Tags

Nuclear target

Expression

Plant

Mutation

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAtU6-sgRNA

Plasmid

#119775

Purpose

Expression of a target sgRNA in potato

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

AtU6-SmR-sgRNA

Use

Tags

Expression

Plant

Mutation

Promoter

AtU6

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)

Plasmid

#160608

Purpose

TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

P35s:dCas9:SRDX:tNOS

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ

Plasmid

#192380

Purpose

To test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pH7WG2B-OsMIR390-B/c

Plasmid

#61465

Purpose

Plant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

Depositor

James Carrington

Article

Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

Insert

OsMIR390-B/c

Use

Tags

Expression

Plant

Mutation

O. sativa MIR390 precursor sequence including a c…

Promoter

OsUBI

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aac

Plasmid

#129685

Purpose

(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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PEP107E

Plasmid

#97393

Purpose

binary construct of Golgi-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Use

Tags

Golgi target

Expression

Plant

Mutation

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pN_35S/mCitrine/P_UBQ10/Derlin1-mOrange2

Plasmid

#118000

Purpose

Co-expression of mCitrine under the control of the CaMV 35S promoter and Derlin1 fused to mOrange2 (an ER-targeting marker protein) under the control of the A. thaliana ubiquitin-10 promoter.

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

Derlin1-mOrange2

Use

Plant/bacteria binary vector

Tags

Expression

Plant

Mutation

Promoter

Ubiquitin-10

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMpGWB106-MTS-Citrine

Plasmid

#79135

Purpose

Binary vectors for expression of Citrine fluorescent protein with mitochondria localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

SD3(1-50)

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ131C2.0

Plasmid

#99886

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

OsU6

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ132C2.0

Plasmid

#99889

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

OsU6

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pAct-AtINT13

Plasmid

#127534

Purpose

Plasmid encodes A. thaliana codon optimized Integrase 13.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 13 coding sequence codon optimized for A. thaliana expression.

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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PEP113E

Plasmid

#97399

Purpose

binary construct of peroxisome-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Use

Tags

Peroxisome target

Expression

Plant

Mutation

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PV225

Plasmid

#132334

Purpose

dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

dCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))

Use

Tags

AT-CBF1

Expression

Plant

Mutation

Codon optimize for expression and stability in Ze…

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PEP114E

Plasmid

#97400

Purpose

binary construct of ER-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Use

Tags

ER target

Expression

Plant

Mutation

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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