Skip to main content
Addgene
Showing: 161 - 200 of 3898 results

  3. pDGE687

    Plasmid
    #167299
    Purpose
    Vector for plant genome editing containing p35S:GFP-Cas9 expression cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 counterselction markers, but not sgRNAs.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    167299-plasmid-map-sequence-id-333326

    Enlarge

  4. pDGB3alpha2_35S:P19:Tnos (GB1203)

    Plasmid
    #68214
    Purpose
    Transcriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoter
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    P19 silencing suppressor
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68214-plasmid-map-sequence-id-119066

    Enlarge

  10. pS-cr:GFP

    Plasmid
    #196295
    Purpose
    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
    Use
    CRISPR
    Tags
    SpeI-DR-BsaI-DR-SpeI
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196295-plasmid-map-sequence-id-390035

    Enlarge

  15. pCBC-DT3T4

    Plasmid
    #50592
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T3, gRNA scaffold, U6-1tplus, U6-26p, T4
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50592-plasmid-map-sequence-id-372989

    Enlarge

  18. CMPE-ePPEplus

    Plasmid
    #205242
    Purpose
    For multiplex plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Article
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Promoter
    Available since
    Oct. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    205242-plasmid-map-sequence-id-408832

    Enlarge

  22. pLX-TRV2

    Plasmid
    #180516
    Purpose
    Agrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and apolylinker
    Depositor
    Jose-Antonio Daros
    Article
    Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:
    Insert
    Tobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S CaMV
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    180516-plasmid-map-sequence-id-355071

    Enlarge

  25. pCHW110032

    Plasmid
    #222438
    Purpose
    Level 0, promoter module (GGAG-AATG) containing CBS4 and CMV1 promoters.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    CBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222438-plasmid-map-sequence-id-454094

    Enlarge

  26. pCHW00011

    Plasmid
    #222439
    Purpose
    Level 0, CDSns module (AATG-TTCG) containing S. cerevisiae CUP2.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    CUP2 (Saccharomyces cerevisiae) (CUP2 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222439-plasmid-map-sequence-id-454078

    Enlarge

  28. pUC19/N-Vc

    Plasmid
    #183157
    Purpose
    Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc.
    Depositor
    Yao-Guang Liu, Qinlong Zhu
    Article
    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183157-plasmid-map-sequence-id-360347

    Enlarge

  29. pSV40-mCherry-P2A-Hygro

    Plasmid
    #106478
    Purpose
    Expression of mCherry and Hygromycin resistance gene from SV40 promoter
    Depositor
    Alex Hewitt
    Inserts
    mCherry
    Hygromycin B resistance
    Use
    Tags
    Expression
    Mammalian and Plant
    Mutation
    Promoter
    SV40 promoter
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106478-plasmid-map-sequence-id-207986

    Enlarge

  34. pDIRECT_25H

    Plasmid
    #91145
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91145-plasmid-map-sequence-id-182281

    Enlarge

  37. pCHW00019

    Plasmid
    #222448
    Purpose
    Level 0, C terminal tag module (TTCG-GCTT) containing GAL4AD-NLS.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    GAL4AD-NLS
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222448-plasmid-map-sequence-id-453911

    Enlarge

  38. pCHW100003

    Plasmid
    #222442
    Purpose
    Level 0, N-terminal tag module (CCAT-AATG) containing mCherry2-HyP5SM.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    mCherry2-HyP5SM
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222442-plasmid-map-sequence-id-454096

    Enlarge

  39. pCHW111114

    Plasmid
    #222459
    Purpose
    Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    CBS4-CMV1 (Cucumber mosaic virus)
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222459-plasmid-map-sequence-id-454092

    Enlarge

  40. pCHW111118

    Plasmid
    #222461
    Purpose
    Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    CBS4-CMV1 (Cucumber Mosaic Virus)
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222461-plasmid-map-sequence-id-454090

    Enlarge

Showing: 161 - 200 of 3898 results