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Plasmid#119774PurposeConversion of C to T in rice plants transformationDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGREAT26
Plasmid#170914PurposeGoldenBraid Double Transcriptional Unit - pEstradiol_Inducible_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpEstradiol_InducibleAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265F2
Plasmid#164722PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-nCas9-NG-CBE_V01 for C-T base editingDepositorInsertA3A(Y130F)-zCas9(D10A)NG-UGI
UseCRISPR; Gateway compatible a3a/y130f-ncas9-ng-cbe…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFG7m34GW
Plasmid#133747PurposeThree-fragment Multisite Gateway vector construction for green fluorescent seed selection in Arabidopsis thalianaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_260d
Plasmid#91126PurposeTransformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterPvUbi2Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB444
Plasmid#74830PurposeGateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ173
Plasmid#99907PurposeExpress CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequenceDepositorInsertpco-dCas9-VP64-T2A-MS2-VP64
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB410
Plasmid#74804PurposeGateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHDE-35S-Cas9-mCherry-UBQ
Plasmid#78932PurposeFor CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEL029
Plasmid#137898PurposeSTU2.0 SpyCas9 RZ system for plant genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL032
Plasmid#137901PurposeSTU2.0 LbCas12a for plant genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL11015
Plasmid#117499PurposeFAST-Red selectable marker Golden Gate Level 1 Position 1DepositorInsertBpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI
UseTagsRFPExpressionPlantMutationPromoterAtOLE1Available sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25F
Plasmid#91143PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ233 (dLbCpf1-SRDX)
Plasmid#86211PurposeDeactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmidDepositorInsertLbCpf1
UseCRISPRTags5' and 3' NLS and SRDX repressorExpressionPlantMutationPromoterAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
AatII-pRPS5A-PTP-3Xflag
Plasmid#189639PurposesTALED for plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUB-Cas9
Plasmid#86556PurposeFor ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)DepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationCas9 is codon optimized for C. reinhardtiiPromoterUBIQUITIN10Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMU-GAr
Plasmid#61172PurposeFluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under AtUBQ10 promoterDepositorInsertGmMAN49-mCherry
UseTagsExpressionPlantMutationPromoterAtUBQ10Available sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCBLgRNA
Plasmid#199726PurposeGateway entry vector for single gRNA under OsU3 promoter.DepositorInsertsgRNA scaffold
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
lvl0 lexA-35S
Plasmid#153407PurposeEstradiol system inducible promoterDepositorInsertlexA-35S promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBUE411-B
Plasmid#198234PurposepPLTP::BBM cassette in pBUE411DepositorInsertpPLTP::BBM cassette
UseCRISPR; Plant binary vectorTagsExpressionPlantMutationPromoterPLTPAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only