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pTC391

Plasmid

#91220

Purpose

protoplast vector expressing gRNA23 targeting tomato ANT1 (with TREX2 expression)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting tomato ANT1

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAtCas9-D10A_1

Plasmid

#91159

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A (nickase)

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pDIRECT_38J

Plasmid

#91154

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTC392

Plasmid

#91223

Purpose

protoplast vector expressing gRNA23 targeting tomato ANT1 (control without TREX2 expression)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting tomato ANT1

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTC366

Plasmid

#91215

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, ribozyme processing)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTaCas9-dead_6

Plasmid

#91175

Purpose

Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_D10A+H840A (dead)

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, H840A

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTaCas9-dead_5

Plasmid

#91174

Purpose

Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_D10A+H840A (dead)

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, H840A

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDIRECT_39J

Plasmid

#91155

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p35S::SISOBIR1-YFPc

Plasmid

#86164

Purpose

SOBIR1 split YFP

Depositor

Silke Robatzek

Article

Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

Insert

SOBIR1 (SOBIR1 Mustard Weed)

Use

Tags

YFPc

Expression

Plant

Mutation

Promoter

35S

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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p35S::AtSOBIR1-YFPc

Plasmid

#86162

Purpose

SOBIR1 split YFP

Depositor

Silke Robatzek

Article

Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

Insert

SOBIR1

Use

Tags

YFPc

Expression

Plant

Mutation

Promoter

35S

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TF101-STTM-miR394

Plasmid

#84813

Purpose

Turning off/down miR394 in Soybean

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM-miR394

Use

Tags

Expression

Plant

Mutation

bp8 is deleted- please see depositor comments bel…

Promoter

unknown

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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TF101-STTM-miR390

Plasmid

#84812

Purpose

Turning off/down miR390 in Soybean

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM-miR390

Use

Tags

Expression

Plant

Mutation

Promoter

unknown

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTX203

Plasmid

#89267

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA02

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsDEP1-gRNA02

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Maize Ubiquitin1 promoter

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTX202

Plasmid

#89266

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA01

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsDEP1-gRNA01

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Maize Ubiquitin1 promoter

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTX200

Plasmid

#89264

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsYSA-gRNA01

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Maize Ubiquitin1 promoter

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTX199

Plasmid

#89263

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA02

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsPDS-gRNA02

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Maize Ubiquitin1 promoter

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM782

Plasmid

#84149

Purpose

target miRNA782 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM782

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM824

Plasmid

#84153

Purpose

target miRNA824 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM824

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM417

Plasmid

#84135

Purpose

target miRNA417 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM417

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM397

Plasmid

#84123

Purpose

target miRNA397 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM397

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge