-
Plasmid#112072PurposeAtDeadCAS9 D10A H840A module CDS1DepositorInsertAtDeadCAS9 D10A H840A
UseCRISPRTagsExpressionPlantMutationPlant codon optimized dCas9 D10A S. pyogenesPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORFE0001
Plasmid#112070PurposeAtCas9 module CDS1DepositorInsertAtCAS9
UseCRISPRTagsExpressionPlantMutationPlant codon optimized Cas9 S. pyogenesPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORFE0014
Plasmid#112076PurposeAtCAS9 nickase D10A module CDS1nsDepositorInsertAtCAS9 D10A without stop codon
UseCRISPRTagsExpressionPlantMutationPlant codon optimized dCas9 D10A S. pyogenesPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pC4H::cogRFP (C90, JBEI-15908)
Plasmid#110143PurposeTransformation and expression of Csy4 and cogDsRed proteins in plantsDepositorInsertCsy4 (ATCS Mustard Weed)
UseBinary vectorTagscogDsRedExpressionBacterial and PlantMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pC4H::cogGFP (C44, JBEI-16338)
Plasmid#110144PurposeTransformation and expression of Csy4 and cogGFP proteins in plantsDepositorInsertCsy4 (ATCS Mustard Weed)
UseTagscogGFPExpressionBacterial and PlantMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
RCH1:GA2ox2-GFP
Plasmid#108567PurposeArabidopsis transformation, expresses GA2ox2 with RCH1 promoter to study expression pattern in rootsDepositorInsertGA2ox2 (GA2OX2 Mustard Weed)
UseTagsExpressionPlantMutationPromoterRCH1Available sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB1alpha12:empty
Plasmid#106208PurposeGoldenBraid level 1 Alpha plant expression vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB1alpha13:empty
Plasmid#106209PurposeGoldenBraid level 1 Alpha plant expression vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB1alpha14:empty
Plasmid#106210PurposeGoldenBraid level 1 Alpha plant expression vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB1alpha13R:empty
Plasmid#106211PurposeGoldenBraid level 1 Alpha plant expression vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD1:P1-MAR
Plasmid#106213PurposeProvides Glycine max P1 matrix attachment region as a level 0 GoldenBraid partDepositorInsertGlycine max P1 matrix attachment region
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD2:SF35
Plasmid#106216PurposeProvides short stuffer fragment of 35bp as a level 0 GoldenBraid partDepositorInsertshort stuffer fragment of 35bp
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD2b:TMV-3NTR
Plasmid#106218PurposeProvides Nos terminator and 3' part of Tobacco mosaic virus (3NTR, ribozyme) as level 0 GoldenBraid part (GCTT-CGCT)DepositorInsertInfectious clone of Tobacco mosaic virus, 3' part
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ATG-3xHA-S11
Plasmid#108238PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (3xHA-S11 as control) in plantsDepositorInsertATG-3xHA-S11
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJKW0457
Plasmid#107586PurposeAtU6-26 sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHDT2:GUS
Plasmid#108444PurposeArabidopsis transformation, expresses HDT2 promoter/GUS fusion to study expression pattern in rootsDepositorInsertHDT2 promoter (HD2B Mustard Weed)
UseTagsGUSExpressionPlantMutationPromoterHDT2Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHDT2:HDT2-GFP
Plasmid#108566PurposeArabidopsis transformation, expresses HDT2/GFP fusion with HDT2 promoter to study expression pattern in rootsDepositorInsertHDT2 (HD2B Mustard Weed)
UseTagsGFPExpressionPlantMutationPromoterHDT2Available sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHDT1:HDT1-GFP
Plasmid#108565PurposeArabidopsis transformation, expresses HDT1/GFP fusion with HDT1 promoter to study expression pattern in rootsDepositorInsertHDT1 (HDA3 Mustard Weed)
UseTagsGFPExpressionPlantMutationPromoterHDT1Available sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGA2ox2:GUS
Plasmid#108564PurposeArabidopsis transformation, expresses GA2ox2 promoter/GUS fusion to study expression pattern in rootsDepositorInsertGA2ox2 promoter (GA2OX2 Mustard Weed)
UseTagsGUSExpressionPlantMutationPromoterGA2ox2Available sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHDT1:GUS
Plasmid#108441PurposeArabidopsis transformation, expresses HDT1 promoter/GUS fusion to study expression pattern in rootsDepositorInsertHDT1 promoter (HDA3 Mustard Weed)
UseTagsGUSExpressionPlantMutationPromoterHDT1Available sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only