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Addgene
Showing: 301 - 320 of 3724 results
  1. pCBC-MT2T3

    Plasmid
    #50594
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Insert
    T2, gRNA scaffold, TaU3t plus, U6-26p, T3
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. pKL2013

    Plasmid
    #172182
    Purpose
    A CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.
    Depositor
    Insert
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pLX-AS

    Plasmid
    #188870
    Purpose
    Compact T-DNA binary vector of the pLX series suitable for assembly of plant virus infectious clones and their Agrobacterium-mediated delivery to plants
    Depositor
    Insert
    none
    Use
    Synthetic Biology; Binary t-dna vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    cauliflower mosaic virus (CaMV) 35S promoter
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pPPED

    Plasmid
    #162468
    Purpose
    Destination Expression vector for dicot plant prime editing
    Depositor
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Use
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine based sequence o…
    Promoter
    2x35S
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. BM00654

    Plasmid
    #155339
    Purpose
    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.
    Depositor
    Insert
    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pCBC-MT3T4

    Plasmid
    #50595
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Insert
    T3, gRNA scaffold, U6-26t plus, OsU3p, T4
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. 35S:YFPc-RabG3b-DN

    Plasmid
    #102374
    Purpose
    fluorescent tagging. Plant expression of YFPc-RabG3b-DN
    Depositor
    Insert
    RabG3B (RABG3B Mustard Weed)
    Use
    Tags
    YFPc
    Expression
    Plant
    Mutation
    T22N
    Promoter
    35S
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. 35S:YFPc-RabG3b-WT

    Plasmid
    #102373
    Purpose
    fluorescent tagging. Plant expression of YFPc-RabG3b-WT
    Depositor
    Insert
    RabG3B (RABG3B Mustard Weed)
    Use
    Tags
    YFPc
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. ePPEplus

    Plasmid
    #205241
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Insert
    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pRGE31

    Plasmid
    #50929
    Purpose
    Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoter
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Rice snoRNA U3 and dual 35S promoter
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 301 - 320 of 3724 results