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Plasmid#136547PurposeLevel1 ZmUBIp::FnCas12a (human codon optimized):NOStDepositorInsertZmUBIp::FnCas12a (human codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH130
Plasmid#136549PurposecrRNA backbone for FnCas12a/CmsI nucleases, combine with OsU6-2 promoter module (pFH37)DepositorInsertcrRNA backbone for FnCas12a/CmsI nucleases, combine with OsU6-2 promoter module (pFH37)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH133
Plasmid#136548PurposeLevel1 ZmUBIp::LbCas12a (human codon optimized):NOStDepositorInsertZmUBIp::LbCas12a (human codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 CA
Plasmid#133264PurposeConstitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.DepositorInsertOsRac1(G19V) (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationOsRac1 G19VPromoterUbiAvailable sinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 DN
Plasmid#133263PurposeDominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assaysDepositorInsertOsRac1(T24N) (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationOsRac1 T24NPromoterUbiAvailable sinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX061
Plasmid#126279PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf04664g02003.1
UseTagsHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX031
Plasmid#126249PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf00073g05010.1
UseTagsHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX006
Plasmid#126224PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf23241g00003.1
UseTagsHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 WT
Plasmid#133262PurposeBiosensor for monitoring activation for rice small GTPase OsRac1DepositorInsertOsRac1 (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationPromoterUbiAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
GS2.1/EC
Plasmid#132568PurposesgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).DepositorInsertegg cell promoter, Cas9-mApple, pds3 sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterA. thaliana egg cell promoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTF101.4
Plasmid#134773PurposeA binary plasmid containing nopaline synthase promoter to drive bialophos resistance gene (bar) for plant transformation.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV246
Plasmid#132337PurposeCasE constitutive expression cassette for Zea maysDepositorInsertCasE
UseTagsExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV248
Plasmid#132339PurposeCasD constitutive expression cassette for Zea maysDepositorInsertCasD
UseTagsExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV259
Plasmid#132340PurposeCasD plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasD-AT-CBF1 (CBF1 Mustard Weed, CasD (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana))
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV503
Plasmid#132342PurposeCasE plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasE-AT-CBF1 (CBF1 Mustard Weed, CasE (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana))
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV504
Plasmid#132343PurposeCasA plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasA-AT-CBF1 (CBF1 Mustard Weed, CasA (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana))
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV384
Plasmid#132344PurposeCas9 DsRed targeting sgRNA expression cassette for Zea maysDepositorInsertCas9 DsRed targeting sgRNA
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV422
Plasmid#132345PurposeSthCascade DsRed targeting crRNA expression cassette for Zea maysDepositorInsertSthCascade DsRed targeting crRNA
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV521
Plasmid#132346PurposeCas9 R promoter targeting sgRNA1 expression cassette for Zea maysDepositorInsertCas9 R promoter targeting sgRNA1
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV523
Plasmid#132347PurposeCas9 R promoter targeting sgRNA2 expression cassette for Zea maysDepositorInsertCas9 R promoter targeting sgRNA2
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only