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Plasmid#138103PurposeGolden Gate entry vector for 4th crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only
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pYPQ233-STU
Plasmid#138111PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated LbCas12a-SRDX repressor without promoter and terminatorDepositorInsertdLbCas12a-SRDX
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239-STU
Plasmid#138112PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized FnCas12a without promoter and terminatorDepositorInsertFnCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-STU-As
Plasmid#138097PurposeGolden Gate entry vector for 2nd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-STU-As
Plasmid#138100PurposeGolden Gate entry vector for 3rd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJIT163-AFID-2
Plasmid#167358PurposeFor inducing predictable deletions in protoplasts and wheat plantsDepositorInsertA3A-Cas9-UDG
UseTagsExpressionPlantMutationWTPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB_3alpha2 OplacI:mini35S:RDF:Tnos (GB1534)
Plasmid#160617PurposeTU for the regulated expression of the PhiC31 phage recombination directionality factor (RDF) gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.DepositorInsertRDF
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromotermini35sAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtIDA10:T35s
Plasmid#164743PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr3g094130.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtCEP12:T35s
Plasmid#164730PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr1g020020.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRWJB006
Plasmid#160113PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoter35SAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRWJB004
Plasmid#160112PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoterUBQ10Available sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtLegin6:T35s
Plasmid#164751PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr3g067445.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtIDA6:T35s
Plasmid#164749PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr1g099210.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtPIP7:T35s
Plasmid#164766PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMT4Noble_020581.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtMEG2:T35s
Plasmid#164754PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMT4Noble_004794.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtFam208_1:T35s
Plasmid#164735PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr7g015340.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtIDA35:T35s
Plasmid#164747PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr7g115360.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG20_FLAG_Blp
Plasmid#159714PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneUseTagsFLAGExpressionPlantMutationPromoterAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG20_Myc_Blp
Plasmid#159716PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneUseTagsMycExpressionPlantMutationPromoterAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtMEG1:T35s
Plasmid#164753PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr7g079830.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only