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LLP721_pSP14

Plasmid

#192434

Purpose

To test the relative expression strength of the Act2 promoter

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP771_L2pV1_NOR_B3RT_FRT_Rluc_2IFB_lacZ

Plasmid

#192431

Purpose

To act as a 2-input state of the NOR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-Rluc-B3RT-FRT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP759_L2pV1_OR-OCS_0I

Plasmid

#192418

Purpose

To act as the 0-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-loxP-OCS-FRT-loxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP760_L2pV1_OR-OCS_TCTP-Flp

Plasmid

#192419

Purpose

To act as a 1-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-loxP-OCS-FRT-loxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ

Plasmid

#192404

Purpose

On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP766_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_2IBF_lacZ

Plasmid

#192410

Purpose

To act as a 2-input state of the A NIMPLY B gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP767_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_2IFB_lacZ

Plasmid

#192411

Purpose

To act as a 2-input state of the A NIMPLY B gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ

Plasmid

#192401

Purpose

Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-Rluc-B3RT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP763_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_0I_lacZ

Plasmid

#192407

Purpose

To act as the 0-input state of the A NIMPLY B gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ

Plasmid

#192400

Purpose

On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-Rluc-B3RT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ

Plasmid

#192402

Purpose

On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ

Plasmid

#192405

Purpose

Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ

Plasmid

#192403

Purpose

Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP739_L2_pV01_0I_B3RT-OCS-Rluc_lacZ

Plasmid

#192383

Purpose

To act as the off state for testing of the alt B3 recombinase.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ

Plasmid

#192381

Purpose

To test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP740_L2pV1_1I_B3RT-OCS-Rluc_TCTP-B3o_lacZ

Plasmid

#192384

Purpose

To act as the on state for testing of the alt B3 recombinase.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP716_L2sV3_NAND_1IF2_FRT_Rluc_FlpF2_C1_lacZ

Plasmid

#192416

Purpose

To act as a 1-input state of the split-NAND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::RlucRluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP717_L2sV3_NAND_2I_FRT_Rluc_Flp-C1_lacZ

Plasmid

#192417

Purpose

To act as the 2-input state of the split-NAND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::RlucRluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP715_L2sV3_NAND_1IF1_FRT_Rluc_FlpF1_C1-NLS_lacZ

Plasmid

#192415

Purpose

To act as a 1-input state of the split-NAND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::RlucRluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP691_L2_2I_FRT-OCS_Rluc_Flp_C1

Plasmid

#192414

Purpose

To act as the 2-input state of the split-AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge