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pMC-1-CmYLCV-6

Plasmid

#176872

Purpose

MoClo Level 0 plasmid, contains CmYLCV-6 promoter

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

CmYLCV

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-mKO2

Plasmid

#182724

Purpose

Transient expression of mKO2 in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

mKO2

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-KO1

Plasmid

#182722

Purpose

Transient expression of KO1 in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

KO1

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-hmKO1

Plasmid

#182732

Purpose

Transient expression of humanized mKO1 in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

hmKO1

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-Citrine

Plasmid

#182581

Purpose

Transient expression of Citrine in plant cell (Cytosol)

Depositor

Yutaka Kodama

Article

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

Citrine

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-TIM21(1–50)-Citrine

Plasmid

#182584

Purpose

Transient expression of TIM21(1–50)-Citrine in plant cell (Mitochondria)

Depositor

Yutaka Kodama

Article

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

TIM21(1–50)

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-KON154-MXMT

Plasmid

#182725

Purpose

Transient expression of KON154-MXMT in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

KON154

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-KOC155-MXMT

Plasmid

#182726

Purpose

Transient expression of KOC155-MXMT in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

KOC155

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-Citrine-PTS

Plasmid

#182585

Purpose

Transient expression of Citrine-PTS in plant cell (Peroxisome)

Depositor

Yutaka Kodama

Article

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

Citrine

Use

Tags

PTS（peroxisomal targeting signal）

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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NCH2exp

Plasmid

#131019

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

Insert

NCH2

Use

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Promoter

P35S

Available since

May 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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MNEAP1exp

Plasmid

#131021

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

Insert

MNEAP1

Use

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Promoter

P35S

Available since

May 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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MKAKU41exp

Plasmid

#131015

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

Insert

MKAKU41

Use

Tags

mCherry-FLAG-HA

Expression

Plant

Mutation

Promoter

P35S

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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NCH1exp

Plasmid

#131017

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

Insert

NCH1

Use

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Promoter

P35S

Available since

May 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pGWT35S-KON168-MXMT

Plasmid

#182727

Purpose

Transient expression of KON168-MXMT in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

KON168

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ230-E795L

Plasmid

#176890

Purpose

Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter

Depositor

Yiping Qi

Article

Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

Insert

LbCas12a-E795L

Use

CRISPR; Gateway compatible lbcpf1 entry clone

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Plant

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF1-L1-dSpRY

Plasmid

#177681

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dCas9-SpRY

Use

CRISPR; Gateway compatible foki-dcas9-spry entry …

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF1-L2-dSpRY

Plasmid

#177682

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dCas9-SpRY

Use

CRISPR; Gateway compatible foki-dcas9-spry entry …

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF-L2-dMb2Cas12a

Plasmid

#177684

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dMb2Cas12a

Use

CRISPR; Gateway compatible foki-dmb2cas12a entry …

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGL193

Plasmid

#180471

Purpose

For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

Depositor

Peggy Lemaux

Article

Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Cas9

Expression

Plant

Mutation

Promoter

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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gMUM2-Citrine

Plasmid

#181965

Purpose

MUM2 protein fused to Citrine, under control of native promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

MUM2 (MUM2 Mustard Weed)

Use

Tags

Citrine YFP

Expression

Plant

Mutation

Promoter

MUM2

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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