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pICSL1P6_AtU626Ter26

Plasmid

#191774

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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CC0101 BFPmut-stop-Bdnf.t1 (pEAQ)

Plasmid

#191173

Purpose

An orthogonal trigger sequence used in plant experiments, derived from the 3' UTR of murine Bdnf

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

Bdnf.t1 (plant)

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ131-RZ-Cas12j2

Plasmid

#189782

Purpose

Golden Gate entry vector to clone the 1st Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ132-RZ-Cas12j2

Plasmid

#189783

Purpose

Golden Gate entry vector to clone the 2nd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ133-RZ-Cas12j2

Plasmid

#189784

Purpose

Golden Gate entry vector to clone the 3rd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ134-RZ-Cas12j2

Plasmid

#189785

Purpose

Golden Gate entry vector to clone the 4th Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265N2

Plasmid

#174001

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-SpRY-UNG

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265O2

Plasmid

#174002

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ131-RZ-Cas12j2

Plasmid

#173927

Purpose

Golden Gate entry vector; empty vector to clone the 1st Cas12j2 crRNA with ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ132-RZ-Cas12j2

Plasmid

#173928

Purpose

Golden Gate entry vector; empty vector to clone the 2nd Cas12j2 crRNA with ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ133-RZ-Cas12j2

Plasmid

#173929

Purpose

Golden Gate entry vector; empty vector to clone the 3rd Cas12j2 crRNA with ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ134-RZ-Cas12j2

Plasmid

#173930

Purpose

Golden Gate entry vector; empty vector to clone the 4th Cas12j2 crRNA with ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265K

Plasmid

#173997

Purpose

Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

UNG-rAPOBEC1(R33A)-zCas9(D10A)

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

R33A in rAPOBEC1 and D10A in zCas9

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265N1

Plasmid

#173998

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-UNG

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265O1

Plasmid

#173999

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-rXRCC1

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265L2

Plasmid

#174000

Purpose

Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYI047

Plasmid

#170843

Purpose

(pFRO6::SMT2-FLAG::tOCS)

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

pFRO6::SMT2-FLAG

Use

Tags

Expression

Plant

Mutation

Promoter

FRO6p

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYI048

Plasmid

#170844

Purpose

(pGH3.17::SMT2-FLAG::tOCS)

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

pGH3.17::SMT2-FLAG

Use

Tags

Expression

Plant

Mutation

Promoter

GH3.17p

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYI001

Plasmid

#170833

Purpose

(GUSPlus::tOCS in pFP100)*

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

GUSPlus::tOCS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYI006

Plasmid

#170840

Purpose

FRO6p::GUSPlus::tOCS in pFP100

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

FRO6p::GUSPlus::tOCS

Use

Tags

Expression

Plant

Mutation

Promoter

FRO6

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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