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LLP745_L2pV1_OR_B3RT_FRT_Rluc_1IB_lacZ

Plasmid

#192389

Purpose

To act as a 1-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ

Plasmid

#192385

Purpose

To test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP746_L2pV1_OR_B3RT_FRT_Rluc_2IFB_lacZ

Plasmid

#192390

Purpose

To act as a 2-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

Plasmid

#192386

Purpose

To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP770_L2pV1_NOR_B3RT_FRT_Rluc_1IB_lacZ

Plasmid

#192430

Purpose

To act as a 1-input state of the NOR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-Rluc-B3RT-FRT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP769_L2pV1_NOR_B3RT_FRT_Rluc_1IF_lacZ

Plasmid

#192429

Purpose

To act as a 1-input state of the NOR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-Rluc-B3RT-FRT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP722_pSP15

Plasmid

#192435

Purpose

To test the relative expression strength of the TCTP1 promoter

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

TCTP1::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP720_pSP13

Plasmid

#192433

Purpose

To test the relative expression strength of the 35S promoter

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

35S::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP721_pSP14

Plasmid

#192434

Purpose

To test the relative expression strength of the Act2 promoter

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP771_L2pV1_NOR_B3RT_FRT_Rluc_2IFB_lacZ

Plasmid

#192431

Purpose

To act as a 2-input state of the NOR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-Rluc-B3RT-FRT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP759_L2pV1_OR-OCS_0I

Plasmid

#192418

Purpose

To act as the 0-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-loxP-OCS-FRT-loxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP760_L2pV1_OR-OCS_TCTP-Flp

Plasmid

#192419

Purpose

To act as a 1-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-loxP-OCS-FRT-loxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ

Plasmid

#192404

Purpose

On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP766_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_2IBF_lacZ

Plasmid

#192410

Purpose

To act as a 2-input state of the A NIMPLY B gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP767_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_2IFB_lacZ

Plasmid

#192411

Purpose

To act as a 2-input state of the A NIMPLY B gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ

Plasmid

#192401

Purpose

Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-Rluc-B3RT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP763_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_0I_lacZ

Plasmid

#192407

Purpose

To act as the 0-input state of the A NIMPLY B gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ

Plasmid

#192400

Purpose

On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-Rluc-B3RT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ

Plasmid

#192402

Purpose

On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ

Plasmid

#192405

Purpose

Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge