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pnCas9-miniSdd3-PBE

Plasmid

#204857

Purpose

For cytosine base editing using miniSdd3 in monocotyledon plants (transient transformation)

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd3-nSpCas9(D10A)-UGI

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A in SpCas9

Promoter

ZmUbi-1

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pZG165

Plasmid

#202584

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pZG161

Plasmid

#202580

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-dADARcd-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pZG160

Plasmid

#202579

Purpose

RNA editing in plants. Plasmid contains extradiol-LIC cassette-dADARcd-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZG159

Plasmid

#202578

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Shim_ProRS_TP_GFP_pK7FWG2

Plasmid

#202651

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al bioRxiv 2023.08.01.551541

Insert

N-terminal transit peptide from putative cytosolic ProRS

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Shim_ThrRS_TP_GFP_pK7FWG2

Plasmid

#202652

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al bioRxiv 2023.08.01.551541

Insert

N-terminal transit peptide from putative cytosolic ThrRS

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Shim_TrpRS_TP_GFP_pK7FWG2

Plasmid

#202653

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al bioRxiv 2023.08.01.551541

Insert

N-terminal transit peptide from putative cytosolic TrpRS

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Silene_conica _GlnRS_MitoTP_pK7FWG2

Plasmid

#202654

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene conica cytosolic GlnRS predicted to be re-targeted to mitochondria

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Silene_conica _LysRS_MitoTP_pK7FWG2

Plasmid

#202655

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene conica cytosolic LysRS predicted to be re-targeted to mitochondria

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Agrostemma_githago _LysRS_MitoTP_pK7FWG2

Plasmid

#202656

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Agrostemma githago cytosolic LysRS predicted to be re-targeted to mitochondria

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Silene_conica _TyrRS_MitoTP_pK7FWG2

Plasmid

#202657

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene conica cytosolic TyrRS predicted to be re-targeted to mitochondria

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Silene_vulgaris _TyrRS_MitoTP_pK7FWG2

Plasmid

#202658

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene vulgaris cytosolic TyrRS predicted to be re-targeted to mitochondria

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Silene_conica _PheRS_MitoTP_pK7FWG2

Plasmid

#202659

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to mito

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Silene_conica _PheRS_PlastidTP_pK7FWG2

Plasmid

#202660

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to plastid

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Arabidopsis_thaliana _PheRS_DualTP_pK7FWG2

Plasmid

#202661

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from organellar Arabidopsis thaliana PheRS known to be dual targeted to plastid and mitochondria

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Shim_CytC_TP_GFP_pK7FWG2

Plasmid

#202646

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al bioRxiv 2023.08.01.551541

Insert

N-terminal transit peptide from putative Cytochrome C

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Shim_AspRS_TP_GFP_pK7FWG2

Plasmid

#202647

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al bioRxiv 2023.08.01.551541

Insert

N-terminal transit peptide from putative cytosolic AspRS

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Shim_GlyRS_TP_GFP_pK7FWG2

Plasmid

#202648

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al bioRxiv 2023.08.01.551541

Insert

N-terminal transit peptide from putative cytosolic GlyRS

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Shim_AsnRS_TP_GFP_pK7FWG2

Plasmid

#202649

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al bioRxiv 2023.08.01.551541

Insert

N-terminal transit peptide from putative cytosolic AsnRS

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge