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Plasmid#158393PurposeGolden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only
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pS-cr:GFP
Plasmid#196295Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSsDepositorInsertFull length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
UseCRISPRTagsSpeI-DR-BsaI-DR-SpeIExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
35S-VP16-GWY/pAM
Plasmid#201234PurposeUsed to produce and express in planta C-terminal fusion proteins with the VP16 activation domainDepositorInsert35S-VP16-GWY
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGD-P1/HC-Pro
Plasmid#196328Purpose35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressorDepositorInserttobacco etch virus P1/HC-Pro
UseTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMKV058
Plasmid#133313PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and WUS2DepositorInsertLuc+. WUS2
UseTagsExpressionPlantMutationPromoterAtUbi10, NosAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL11056
Plasmid#68258PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plantsDepositorInsertPromoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGW-TurboID-GOI
Plasmid#209380PurposeGateway compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
ePPE
Plasmid#183095PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertNls-nCas9-NC-nls-MLV-Nls
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9PromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL-TRBO
Plasmid#80082PurposeAn improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGD-γb
Plasmid#196327Purpose35S promoter-driven expression of the barley stripe mosaic virus γb RNA silencing suppressorDepositorInsertbarley stripe mosaic virus γb
UseTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGE670
Plasmid#153236PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus
Plasmid#61629PurposeR2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoterDepositorInsertsmDII
ntdTomato
DII
n3xVenus
UseTagsExpressionPlantMutationPromoterRPS5AAvailable sinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMM135
Plasmid#127216PurposeBeYDV replicon with constitutive Luc expression, WUS2, sgRNA targeting PDSDepositorInsertLuc, WUS2, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)
Plasmid#160626PurposeTU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domainsDepositorInsertP35s-dCas9:TV-Tnos
UseTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB401
Plasmid#74795PurposeGateway cloning compatible binary vector for simple cloning of gene.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNo PromoterAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pS-sg:GFP
Plasmid#196296Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSsDepositorInsertFull length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
UseCRISPRTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKI1.1R
Plasmid#85808PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.DepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAG and NLSExpressionPlantMutationPromoterAtRPS5AAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE687
Plasmid#167299PurposeVector for plant genome editing containing p35S:GFP-Cas9 expression cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 counterselction markers, but not sgRNAs.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos
Plasmid#211842PurposePlant transient expression cassette for a Dexamethasone-inducible E3-DART ligaseDepositorInsertsLRR-NEL
GAL4-VP16-GR
UseSynthetic BiologyTagsHA, Myc, and mCherryExpressionPlantMutationPromoter6xUAS-35Smini and pUBQ10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-Cas9
Plasmid#196325Purpose35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GPDepositorInsertFull length TSWV M antigenome encoding SpCas9 in place of viral GP
UseCRISPRTags3×flagExpressionPlantMutationSee depositor comments belowPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only