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Plasmid#195938PurposePhiC31-intein(DnaE-Npu) and Intein(DnaE-Ssp)-PhiC31 : pUBQ10-PI-tUBQ1-p35S-IP-t35SDepositorInsertpUBQ10-PI-tUBQ1-p35S-IP-t35S
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1P27
Plasmid#195929PurposeLevel 1 PhiC31 cassette with constitutive promoter: p35S-UbArg-PhiC31NLS-tUBQ1DepositorInsertp35S-UbArg-PhiC31NLS-tUBQ1
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCLV3Cas-LT2
Plasmid#210550PurposeBinary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporterDepositorInsertCLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT2::mCherrySSM
UseTagsExpressionPlantMutation1bp insertion after AAVS1 target sequence that di…PromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
ipGL2Cas
Plasmid#210555PurposeBinary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by GL2 promoter.DepositorInsertGL2p::XVE::UBQ10t::LexA::SpCas9::PEA3AT::VenusSSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
ipYUC2Cas-LT1
Plasmid#210559PurposeBinary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by M. polymorpha YUC2 promoter.DepositorInsertpMpEF1::XVE::UBQ10t::LexA::SpCas9::PEA3AT::pU6::AAVS1gRNA::pMpEF1::LT1::Hygromycin
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1Cas-LT1
Plasmid#210556PurposeBinary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1pDepositorInsertpMpEF1::Cas9::PEA3AT::U6p::AAVS1gRNA::EF1p::LT1::Hygromycin
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB605-GBSSI
Plasmid#213867PurposeBinary vector for the expression of GBBSI fused to the sGFP in plants (for Agrobacterium-mediated genetic transformation.)DepositorInsertGRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB560-GBSSI
Plasmid#213866PurposeBinary vector for the expression of GBBSI fused to the tagRFP in plants (for Agrobacterium-mediated genetic transformation.)DepositorInsertGRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S
Plasmid#211839PurposePlant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.DepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationR181A R185APromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos
Plasmid#211841PurposePlant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantMutationPromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos
Plasmid#211844PurposePlant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantMutationC492APromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-Ter
Plasmid#211847PurposePlant transient expression cassette for RHD6 fused with GFP and a mutant form of the HR1b degronDepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationR181A R185APromoter35SAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:nnLuz-I{PIV-216}:NosT
Plasmid#212185PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the truncated 35sCAMV promoter and Tomato mosaic virus UTRDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/o35s[TMV]:nnLuz-I{PIV-216}:NosT
Plasmid#212186PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the original 35sCAMV promoter and Tomato mosaic virus UTRDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…Promoteroriginal 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[]:nnLuz-I{PIV-216}:NosT
Plasmid#212187PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the truncated 35sCAMV promoterDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:nnLuz:NosT
Plasmid#212188PurposeThis binary vector expresses mushroom luciferase (nnLuz) with the truncated 35sCAMV promoter and Tomato mosaic virus UTRDepositorInsertnnLuz
UseTagsExpressionPlantMutationPromotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/o35s[TMV]:nnLuz:NosT
Plasmid#212189PurposeThis binary vector expresses mushroom luciferase (nnLuz) with the original 35sCAMV promoter and Tomato mosaic virus UTRDepositorInsertnnLuz
UseTagsExpressionPlantMutationPromoteroriginal 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-I{PDK-198}:NosT
Plasmid#212170PurposeThis binary vector expresses mNeonGreen containing the castor bean catalase (PDK) intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PDK-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PDK int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[]:mNeonGreen-CI{PIV-198}:NosT
Plasmid#212171PurposeThis binary vector expresses mNeonGreen containing the PIV intron (198bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoterDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-CI{PIV-198}:NosT
Plasmid#212172PurposeThis binary vector expresses mNeonGreen containing the PIV intron (198bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only