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Plasmid#218566PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
UseTagsExpressionPlantMutationDeletion genomic sequence from 58nt-147ntPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1-2:GFP
Plasmid#218565PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1:GFP
Plasmid#218564PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pNK7183
Plasmid#219760PurposeMoClo-compatible Level 1 for expression PzPKS2 in plants (N.benthamiana, BY2 cells)DepositorInsertPzPKS2
UseTagsExpressionPlantMutationPromoterp35s_0.4kb - 5'UTR TMV omegaAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7187
Plasmid#219761PurposeMoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)DepositorInsertPpASCL
UseTagsExpressionPlantMutationPromoterp35s_0.4kb - 5'UTR TMV omegaAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7180
Plasmid#219762PurposeMoClo-compatible Level 1 for expression HmS in plants (N.benthamiana, BY2 cells)DepositorInsertHmS
UseTagsExpressionPlantMutationPromoterp35s_0.4kb - 5'UTR TMV omegaAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7191
Plasmid#219764PurposeMoClo-compatible Level M for expression PpASCL in plants (N.benthamiana, BY2 cells)DepositorInsertPpASCL
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
p4P1R_EC1.2en-EC1.1p
Plasmid#213912PurposeEntry clone containing Egg cell-specific promoter (EC1.1p) fused to EC1.2 enhancer (EC1.2en). For use in plants and compatible with the MultiSite Gateway systemDepositorInsertEC1.2en-EC1.1p
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pECNUS3
Plasmid#184886PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pECNUS1
Plasmid#184884PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ121-Split-TALED
Plasmid#218171PurposeExpress plastid TALE-linked deaminases (TALED), composed of TALE, split DddA originated from Burkholderia cenocepacia, and an engineered deoxyadenosine deaminase derived from the E. coli TadA proteinDepositorInsertTALE-Intron-N-DddAtox half (G1397N)-T2A-TALE-C-DddAtox half (G1397C)-TadA8e
UsePlastid base editingTagsExpressionPlantMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ121-mTCBE
Plasmid#218172PurposeExpress plastid monomeric TALE-linked CBE, composed of TALE, a catalytically impaired, full length DddA variant (E1347A), a human APOBEC3A variant (hA3A-Y130F), and UGIDepositorInsertTALE-Intron-hA3A-Y130F-DddA full (E1347A)-UGI
UsePlastid base editingTagsExpressionPlantMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ121-mTCABE-T
Plasmid#218173PurposeExpress plastid monomeric TALE-linked CABE-T, composed of TALE, a catalytically impaired, full length DddA variant (E1347A), a TadA-derived deaminase, and UGIDepositorInsertTALE-Intron-TadAC-DddA full (E1347A)-UGI
UsePlastid base editingTagsExpressionPlantMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
PYPQ121-DdCBE
Plasmid#218174PurposeExpress plastid DdCBE, composed of TALE, split DddA originated from Burkholderia cenocepacia and UGIsDepositorInsertTALE-Intron-N-DddAtox half (G1397N)-UGI-T2A-TALE-C-DddAtox half (G1397C)-UGI
UsePlastid base editingTagsExpressionPlantMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBi2FC-N-MPK5-P2A:mCherry
Plasmid#216136PurposeControls for Bicistronic BiFCDepositorInsertMPK5 (MPK5 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBi2FC-N-MPK8-P2A:mCherry
Plasmid#216138PurposeControls for Bicistronic BiFCDepositorInsertMPK8
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBi2FC-N-MPK12-P2A:mCherry
Plasmid#216139PurposeControls for Bicistronic BiFCDepositorInsertMPK12 (MPK12 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBi2FC-C-MGGAGM-P2A:mTagBFP2
Plasmid#216141PurposeVector control for Bicistronic BiFCDepositorInsertMGGAGM Linker
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1P137 - pAHP6-PhiC31-NLS-tUBQ1
Plasmid#219702PurposeLevel 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integraseDepositorInsertpAHP6:PhiC31-NLS:tUBQ1
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only