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Plasmid#222478PurposeGateway vector for 35s promoter-driven expression in Marchantia polymorpha carrying a sulfadiazine selection marker.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only
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pMpGWBs00
Plasmid#222476PurposeMarchantia polymorpha transformation vector carrying sulfadiazine selection marker.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[]:mCherry-I{PIV-180}:NosT
Plasmid#212179PurposeThis binary vector expresses mCherry containing the PIV intron (180bp into the gene) with the truncated 35sCAMV promoterDepositorInsertmCherry-I{PIV-180}
UseTagsExpressionPlantMutationTruncated mCherry, missing the first 9 amino acid…Promotertruncated 35sCaMVAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/MtHp:nnLuz-I{PIV-216}:NosT
Plasmid#212192PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the strong Medicago trunculata MtHP promoter and UTRDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…PromoterMedicago trunculata MtHP promoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/SCBV:nnLuz-I{PIV-216}:NosT
Plasmid#212190PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the Sugarcane bacilliform virus (SCBV) promoter and UTRDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…PromoterSugarcane Bacilliform VirusAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[]:nnLuz:NosT
Plasmid#212183PurposeThis binary vector expresses mushroom luciferase (nnLuz) with the truncated 35sCAMV promoterDepositorInsertnnLuz
UseTagsExpressionPlantMutationcodon modified for Physcomitrella patens expressi…Promotertruncated 35sCaMVAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/Os-ubi:nnLuz-I{PIV-216}:NosT
Plasmid#212191PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the rice ubiquitin (R. ubi) promoter and UTRDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…PromoterRice ubiquitinAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCambia-dLB-kanR-GFP
Plasmid#223177PurposePlant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left borderDepositorTypeEmpty backboneUseTi plasmid for agrobacteriumTagsExpressionPlantMutationPromoterAvailable sinceAug. 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pECNUS2
Plasmid#184885PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT9054
Plasmid#219430PurposeTranscriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCSDepositorInsertLB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
UseCRISPR; Moclo compatible level 1 module (position…TagsPapE-4LF2ExpressionPlantMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
int>pro::GFP
Plasmid#218576PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
UseTagsExpressionPlantMutationInsert genomic sequence 41-372nt to -46nt upstrea…PromoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1-2(intΔNAC2):GFP
Plasmid#218574PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
UseTagsExpressionPlantMutationMutation genomic sequence 206nt, 207nt from CT to…PromoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)
Plasmid#219690PurposeLevel 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PsB/p35S (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)
Plasmid#219689PurposeLevel 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PsB (mtagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)
Plasmid#219688PurposeLevel 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2BP/p35S (BFP, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
Plasmid#219687PurposeLevel 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)
Plasmid#219686PurposeLevel 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PB/p35S (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK880
Plasmid#219726PurposeMoClo-compatible Level 0 - promoter vector carrying promoter MinSyn105DepositorInsertpromoter MinSyn105
UseSynthetic BiologyTagsExpressionPlantMutationPromoterMinSyn105Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK879
Plasmid#219727PurposeMoClo-compatible Level 0 - promoter vector carrying promoter MinSyn108DepositorInsertpromoter MinSyn108
UseSynthetic BiologyTagsExpressionPlantMutationPromoterMinSyn108Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK887
Plasmid#219728PurposeMoClo-compatible Level 0 - promoter vector carrying promoter MinSyn110DepositorInsertpromoter MinSyn110
UseSynthetic BiologyTagsExpressionPlantMutationPromoterMinSyn110Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only