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Plasmid#74873PurposeGateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNo PromoterAvailable sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only
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pDGB3_alpha1-AtUBQ10:HR1b-GFP-T35S
Plasmid#211837PurposePlant transient expression cassette for HR1b-GFP fusion under UBQ10 promoter.DepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationPromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTF101.1
Plasmid#134770PurposeA binary plasmid containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.DepositorArticleTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCBC-MT2T3
Plasmid#50594PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT2, gRNA scaffold, TaU3t plus, U6-26p, T3
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB107
Plasmid#68561PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJJB296
Plasmid#107691Purpose35SPromoter-LwaCas13a-HSPTerminatorDepositorInsertCas13a
UseTagsExpressionPlantMutationPromoter35SAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE
Plasmid#119768PurposeConversion of C to T in wheat plants or monocotyledons protoplastsDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A and E945K in wild type …Promotermaize Ubiquitin-1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA-FLS2prom::FLS2-3xmyc-GFP
Plasmid#86157PurposeFLS2-GFP expresssionDepositorInsertFLS2 (FLS2 Mustard Weed)
UseTags3xmyc and GFPExpressionPlantMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJY-RpABE
Plasmid#112872Purposebinary vector for ABE expression in ArabidopsisDepositorInsertsRPS5A promoter
ABE7.10
U6-sgRNA cassette
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNC-Cam1304-MCS
Plasmid#193387PurposeNimble cloning-compatible vector for plant ectopic expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEL685
Plasmid#205592PurposeNEPE for prime editing in riceDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterZmUbiAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMU-ERr
Plasmid#61170PurposeFluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoterDepositorInsertAtWAK2-mCherry-HDEL
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceApril 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10-GW
Plasmid#209370PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by UBQ10 promoterDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-tRNA2.0
Plasmid#158394PurposeGolden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDicAID_nCas9-PmCDA_NptII_Della
Plasmid#91694PurposeDicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDellaDepositorInsertSpCas9
UseTagsPmCDA1ExpressionPlantMutationD10A for nickase Cas9PromoterPcUbiAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGE010
Plasmid#71536PurposeFor CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB337
Plasmid#68061PurposeGateway-based vector for Marchantia polymorpha to express a floxed gene, with an expression cassette for Cre-glucocorticoid receptor fusion protein under the control of heat-inducible promoterDepositorTypeEmpty backboneUseCre/LoxTagsExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMU-ACTLr
Plasmid#61193PurposeFluorescent reporter for F-actin LifeAct-mCherry expressed under AtUBQ10 promoterDepositorInsertLifeAct-mCherry
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-Cre-GR-9
Plasmid#197723PurposePhytobrick (MoClo) Level 0 PartDepositorInsertCre recombinase with St-LS1 intron fused to glucocorticoid receptor
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH-STEME-1-esgRNA
Plasmid#138135PurposeTargeted simultaneous C-to-T and A-to-G in riceDepositorInsertAPOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos
Plasmid#211842PurposePlant transient expression cassette for a Dexamethasone-inducible E3-DART ligaseDepositorInsertsLRR-NEL
GAL4-VP16-GR
UseSynthetic BiologyTagsHA, Myc, and mCherryExpressionPlantMutationPromoter6xUAS-35Smini and pUBQ10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiFCt-2in1-CN
Plasmid#105113Purposeratiometric BiFC analysis in plants (transient transformation)DepositorTypeEmpty backboneUseTagscYFP-HA and nYFP-MycExpressionPlantMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDe-CAS9
Plasmid#61433PurposeBinary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequenceDepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationPromoterPcUBI4-2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB441
Plasmid#74827PurposeGateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ173
Plasmid#99907PurposeExpress CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequenceDepositorInsertpco-dCas9-VP64-T2A-MS2-VP64
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB409
Plasmid#74803PurposeGateway cloning compatible binary vector for N-terminal fusion with 6xHis (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMKV058
Plasmid#133313PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and WUS2DepositorInsertLuc+. WUS2
UseTagsExpressionPlantMutationPromoterAtUbi10, NosAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB115
Plasmid#68569PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged Citrine (Citri…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-WDV-LRS-L-11
Plasmid#197720PurposePhytobrick (MoClo) Level 0 PartDepositorInsertWDV LRSL in c-sense with GG expansion to v-sense ORF position
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB509
Plasmid#74851PurposeGateway cloning compatible binary vector for N-terminal fusion with 6xHis (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
SunTag-FWAgRNA-4-22aa-TET1cd
Plasmid#106435PurposeSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoterDepositorInsertgRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-E795L
Plasmid#176890PurposeGateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoterDepositorInsertLbCas12a-E795L
UseCRISPR; Gateway compatible lbcpf1 entry cloneTagsNucleoplasmin NLS and SV40 NLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMU-MTUBr
Plasmid#61196PurposeFluorescent reporter for microtubules mCherry-MAP4-MBD expressed under AtUBQ10 promoterDepositorInsertMAP-MBD
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSfinx-mYFP
Plasmid#216206PurposeA simplified disease resistance assay using YFP-expressing Potato Virus X in N. benthamianaDepositorInsertmonomeric yellow fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd gRNA4 (FWA)
Plasmid#115486PurposeCRISPR-Cas9 SunTag system to target NtDRMcd to the FWA locus with a single guide RNADepositorInsertg4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXSE901BG
Plasmid#91714PurposeCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistanceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9Pubi-B
Plasmid#66188Purposeexpression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotermaize ubiquitin promoter (Pubi)Available sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPY22-mNeonGreen-Hyg
Plasmid#137082PurposeCodon-optimized mNeonGreen for expression in Arabidopsis thaliana and Physcomitrella patensDepositorInsertmNeonGreen-Flag
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH-nCas9-PPE-V2
Plasmid#170131PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertnCas9(H840A)-M-MLV
UseCRISPRTagsExpressionPlantMutationH840A for Cas9; D200N, T306K, W313F, T330P and L…Promotermaize Ubiquitin-1, OsU3Available sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL4723-P1
Plasmid#86173PurposeLevelP cloning VectorDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only