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Plasmid#141558PurposeLentiviral vector for overexpressing the MYCL transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYCL (MYCL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_APEX_FLAG_Ct
Plasmid#194069Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsFLAG-APEX-V5ExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1273
Plasmid#142774PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertDPF2 (DPF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2678
Plasmid#144065PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPEG3 (PEG3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
P2RY2-Tango
Plasmid#66474PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertP2RY2 (P2RY2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-Sharpin
Plasmid#195092PurposeGene Overexpression of Human SHARPINDepositorInsertSHARPIN (SHARPIN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LbABE8e(TadA-8e V106W)
Plasmid#138508PurposeA-to-G base editorDepositorInsertecTadA(8e V106W)-dLbCas12a
UseTagsExpressionMammalianMutationTadA-8e V106WPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGlnA-Ypet
Plasmid#98278Purposemammalian expression of GlnA-YpetDepositorInsertGlnA
UseTagsYpetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
Multiplex-CRISPR-miR-200FAM-T2A-GFP
Plasmid#179856Purposeto delete miR200 family genes including miR-141, miR-200c, miR-429 and miR-200bDepositorInsertUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.RGS4.C-HA
Plasmid#154847PurposeExpresses murine RGS4 protein with HA-tag at C-terminusDepositorInsertRegulator of G protein Signaling 4 (Rgs4 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2959
Plasmid#144435PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKAT2A (KAT2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD474N-EGFP
Plasmid#68847Purposemutated OPTN was inserted into pEGFP-N1 backboneDepositorInsertoptineurin (OPTN Human)
UseTagsEGFPExpressionMammalianMutationD474N (not linked to any diseases)PromoterCMVAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt11
Plasmid#184512PurposeMammalian expression of Synaptotagmin 11 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 11 (Syt11 Mouse)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-INPP5B
Plasmid#171947PurposeExpresses INPP5B in mammalian cellsDepositorInsertINPP5B (INPP5B Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD005 pcDNA Golden Gate
Plasmid#138749PurposeVector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Integrin beta1(1-455)-tev-BHCys
Plasmid#27315DepositorInsertintegrin beta1 (ITGB1 Human)
UseTagsExpressionMammalianMutationbeta1(1-455)+GG+TEV+GGKN+BHCys+TG+His6PromoterAvailable sinceApril 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pR96L-EGFP
Plasmid#68846Purposemutated OPTN was inserted into pEGFP-N1 backboneDepositorInsertoptineurin (OPTN Human)
UseTagsEGFPExpressionMammalianMutationR96L, associated with ALSPromoterCMVAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF1679
Plasmid#143835PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRUNX1T1 (RUNX1T1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2680
Plasmid#144144PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPEG3 (PEG3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L1-PGK-TKNeo-L2
Plasmid#141008PurposeMutisite Gateway mediated vector constructionDepositorInsertTKNeo
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only