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Addgene
Showing: 1281 - 1300 of 69315 results
  1. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid
    #71685
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
    Depositor
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3.1+ SMN1 myc HIS

    Plasmid
    #71687
    Purpose
    Mammalian expression of SMN1
    Depositor
    Insert
    SMN1 (SMN1 Human)
    Use
    Tags
    HIS and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  3. pCW57-MCS1-2A-MCS2

    Plasmid
    #71782
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Depositor
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pCW57-GFP-2A-MCS

    Plasmid
    #71783
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.
    Depositor
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pFCK_smFP FLAG _ Bright

    Plasmid
    #71815
    Purpose
    Fluorescent version of smFP FLAG
    Depositor
    Insert
    smFP FLAG _ Bright
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pCAGmRuby2smFPMyc

    Plasmid
    #71816
    Purpose
    encodes multiple copies of an epitope tag Myc ( not detected by anti GFP ab)
    Depositor
    Insert
    mRuby2_smFP_Myc
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
  7. pCAGmRuby2smFPV5

    Plasmid
    #71817
    Purpose
    encodes multiple copies of an epitope tag V5( not detected by anti GFP ab)
    Depositor
    Insert
    mRuby2_smFP_V5
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
Showing: 1281 - 1300 of 69315 results