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pmEGFP_a_tubulin_IRES_puro2b

Plasmid

#21042

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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lentiCas9-EGFP

Plasmid

#63592

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

Depositor

Phil Sharp, Feng Zhang

Article

Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

Inserts

Cas9
EGFP

Use

CRISPR and Lentiviral

Tags

FLAG and P2A

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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pWN_U6-B2M-miniGag-Cas9

Plasmid

#228958

Purpose

miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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ALIX-mNeonGreen

Plasmid

#191191

Purpose

Full-length ALIX with C-terminal mNeonGreen tag.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (PDCD6IP Human, Synthetic)

Use

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP704nano

Plasmid

#206192

Purpose

Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP704nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

Insert

miRFP704nano-human alpha-tubulin

Use

Tags

miRFP704nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLX303-ZIM3-KRAB-dCas9

Plasmid

#154472

Purpose

Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Synthetic)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 puro Nodamura B2

Plasmid

#17228

Depositor

Christopher Sullivan

Article

Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

Insert

Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)

Use

Tags

Expression

Mammalian

Mutation

2 silent point mutations

Promoter

Available since

June 22, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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YAP1 (S6A) - V5 in pLX304

Plasmid

#42562

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S61A, S109A, S127A, S128A, S131A, S163A, S164A, S…

Promoter

CMV

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-mTagBFP2

Plasmid

#122373

Purpose

Expresses mTagBFP2 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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tetO.Nfib.Hygro

Plasmid

#117271

Purpose

Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

Depositor

Henrik Ahlenius

Article

Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

Insert

Nfib (Nfib Mouse)

Use

Lentiviral

Tags

IRES and hygromycin resistant cassette (C termina…

Expression

Mammalian

Mutation

Promoter

tet-on

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSCAR_Cas9-blast_GFP

Plasmid

#162074

Purpose

3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Inserts

Cas9-2a-blasticidin S deaminase
EGFP

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

codon-optimized for expression in M. musculus

Promoter

EF1a and sv40

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-OMMjRCaMP-P2A-mito4xGCaMP6f

Plasmid

#127872

Purpose

Dual expression vector for mitochondrial matrix and outer membrane Ca2+ sensors

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

Outer membrane localized jRCaMP1b - P2A - mito4xGCaMP6f

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-Broccoli

Plasmid

#124360

Purpose

Overexpression of circular RNA (Broccoli)

Depositor

Samie Jaffrey

Article

Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

Insert

Tornado-Broccoli

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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evoBxb1

Plasmid

#222338

Purpose

Plasmid for expressing evolved Bxb1 in the mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved Bxb1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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tetO.Sox9.Puro

Plasmid

#117269

Purpose

Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

Depositor

Henrik Ahlenius

Article

Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Tags

T2A and puromycin resistant cassette (C terminal …

Expression

Mammalian

Mutation

Promoter

tet-on

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-Mito4x-GCaMP6f

Plasmid

#127870

Purpose

Fluorescent reporter for mitochondrial matrix Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

4 repeats of COX8 signal peptide plus GCaMP6f

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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PiggyBac dCas9-VPR

Plasmid

#191269

Purpose

piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Inserts

VPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL_SRSF2_WT_mCherry

Plasmid

#84020

Purpose

expression of SRSF2 in mammalian cells

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-GRP78/BiP

Plasmid

#32701

Depositor

Richard C. Austin

Article

Werstuck et al J Clin Invest. 2001 May;107(10):1263-73.

Insert

GRP78/BiP (HSPA5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 15, 2013

Availability

Academic Institutions and Nonprofits only

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BPK1520

Plasmid

#65777

Purpose

Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SpCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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