Addgene

Showing: 941 - 960 of 71600 results

Results per page:

Sort results by:

murine TCR OTII-2A.pMIG II

Plasmid

#52112

Purpose

retroviral expression of both TCRalpha and TCRbeta using a single vector

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Inserts

T cell receptor alpha chain (Tcra Mouse)
T cell receptor beta chain (Tcrb Mouse)

Use

Retroviral

Tags

P2A (from porcine teschovirus-1)

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-EGFP-NR1 wt

Plasmid

#45446

Depositor

Robert Malinow, Andres Barria

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR1 (Grin1 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-D1R

Plasmid

#89160

Purpose

Express vaccinia virus D1R in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Vaccinia virus D1R

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promotor

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-D12L

Plasmid

#89161

Purpose

Express vaccinia virus D12L in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Vaccinia virus D12L

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promotor

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
phU6-gRNA

Plasmid

#53188

Purpose

Expresses the S. pyogenes sgRNA from the human U6 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
gag-P3-pol

Plasmid

#211374

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-P3-pol

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Frankenbody-FLAG-mEGFP

Plasmid

#175293

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mEGFP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Lamp1-mNeonGreen

Plasmid

#98882

Purpose

In vivo visualization of lysosomes (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

Lamp1

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-mCherry-flex-dtA

Plasmid

#58536

Purpose

Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

Depositor

Naoshige Uchida

Article

Wu et al Nature. 2014 May 15;509(7500):325-30. do

Insert

dtA (DIP_RS18250 )

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1/PD-L1

Plasmid

#121478

Purpose

expression of human PD-L1 in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-TFEB

Plasmid

#38119

Purpose

Mammalian expression of Transcription factor EB fused to EGFP

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-C1-RanQ69L

Plasmid

#30309

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

RanQ69L (RAN N/A)

Use

Tags

FLAG-Cherry

Expression

Mammalian

Mutation

Glutamine 69 is changed to Leucine

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-Myc-TEAD1

Plasmid

#33109

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD1 (TEAD1 )

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
attB-puro donor.dna

Plasmid

#181923

Purpose

attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attB-EGFP-EF1α-PuroR-T2A-TagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pmScarlet-I3_C1

Plasmid

#189756

Purpose

C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-mSTING

Plasmid

#214157

Purpose

Transient expression of STING

Depositor

Nan Yan

Article

Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

Insert

STING (Sting1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

Plasmid

#190033

Purpose

vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters

Depositor

Huawei Tong

Article

Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

Insert

humanized hfCas13X

Use

Tags

Expression

Mammalian

Mutation

Y672A,Y676A

Promoter

CBh, SV40, hU6

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX_305_Ovalbumin

Plasmid

#184924

Purpose

Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

Depositor

Arlene Sharpe

Article

Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

Insert

Ovalbumin (OVAL Chicken)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Our vector has an A188T mutation that does not im…

Promoter

PGK

Available since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-NONO

Plasmid

#127655

Purpose

Over-express HA-tagged human NONO

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

Insert

NONO (NONO Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gag-PE

Plasmid

#211371

Purpose

Packaging plasmid for v3 PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-PE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge