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Plasmid#89540PurposeCyan color variant of bright luminescent protein, fused to clathrin light chainDepositorInsertCeNL
UseTagsClathrin light chainExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC22A17_STOP
Plasmid#161201PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC22A17 (SLC22A17 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NES-mut
Plasmid#42046DepositorInsertTNRC6A (TNRC6A Human)
UseTagsFLAG, HA, and SBPExpressionMammalianMutationF951A, F955A, I958A, F960APromoterAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NLS-mut
Plasmid#42045DepositorInsertTNRC6A (TNRC6A Human)
UseTagsFLAG, HA, and SBPExpressionMammalianMutationR930A, R931A, R932A, R934APromoterAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWZL Hygro-NFLAG TRF1 deltaA
Plasmid#16278DepositorInserthTRF1 (TERF1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationDeleted acidic domain (aa1-66)PromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pWZL Hygro-NFLAG TRF1 deltaM
Plasmid#16281DepositorInserthTRF1 (TERF1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationDeleted myb domain (aa379-439)PromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pWZL Hygro-NFLAG TRF1 deltaA deltaM
Plasmid#16297DepositorInserthTRF1 (TERF1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationDeleted acidic domain (aa1-66) and myb domain (aa…PromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE-U2AF2-TurboID-HA-rtTA
Plasmid#226997PurposeIntegrative plasmid to express U2AF2-TurboID in mammalian cellsDepositorInsertU2 small nuclear RNA auxiliary factor 2 (U2AF2 Human)
UseTagsTurboID-HAExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cre-MS2_UbC-GFP (Cre_GFP)
Plasmid#219536Purposea. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotorDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX552-EF1a-DIO-mcherry(with gRNA scaffold)
Plasmid#199581PurposeExpresses mCherry in a Cre-dependent mannerDepositorInsertmCherry
UseAAV, CRISPR, and Cre/LoxTagsExpressionMammalianMutationPromoterEF1a intron A and U6Available sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMMS22L-siR3
Plasmid#221013Purposemammalian expression vector of myc-Flag tagged MMS22L siRNA resistantDepositorInsertMMS22L (MMS22L Human)
UseTagsFlag and mycExpressionMammalianMutationsilent mutations MMS22L: 5'-aaAacGtgTtgCtgTg…PromoterCMVAvailable sinceJuly 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
mAIRN HO
Plasmid#207411PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).DepositorInsertmAIRN
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mAIRN CD
Plasmid#207412PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).DepositorInsertmAIRN
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_SMU1:1-513
Plasmid#211114PurposeMammalian expression of full-length human SMU1. N-terminal HiBiT tag.DepositorInsertSMU1:M1-P513 (SMU1 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMV-BC0527-luc2
Plasmid#214657Purposebarcoded assay, Ca2+ sensorDepositorInsert6x clustered NFAT element with barcode 0527
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2353
Plasmid#144017PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF12 (ZNF12 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNXF-1-5xmut_O
Plasmid#147116PurposeMammalian Expression of HsNxf-1-5xmutDepositorInsertHsNxf-1-5xmut (NXF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP
Plasmid#195505PurposeT-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17DepositorInsertpT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
UseCRISPR; Donor vectorTagsmRuby-3xFLAG-NLS-3xSV40-poly(A)ExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSPL3-hRPH3A_c.1853Gmut
Plasmid#190479Purposeminigene assayDepositorInsertRPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
UseTagsExpressionMammalianMutationc.1853A>GPromoterSV40Available sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBW2602_pCAG-PV1-iCre-N256-L1-FRB-NLS-BGHpA
Plasmid#108735PurposeExpresses N-terminus to aa256 of Cre recombinase fused to FRB CIDDepositorInsertCre recombinase fragment N-terminus to aa256
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only