-
Plasmid#207414PurposeMammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).DepositorInsertECFP
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2279
Plasmid#144331PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPRDM2 (PRDM2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHA-N1
Plasmid#86049PurposeA mammalian expression vector with a HA-tag at C-terminus.DepositorTypeEmpty backboneUseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-Renilla
Plasmid#87065PurposeMammalian Gateway-compatible expression vector backbone with a C-terminal Renilla luciferaseDepositorTypeEmpty backboneUseTagsRenilla luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
EK0211 CMV-TO-BFPmut-stop-t17d9b (FLP-IN)
Plasmid#191139PurposeExpression of Trigger 2 in 3' UTR of an inactive BFP in mammalian cells. Used as second input in OR, AND gatesDepositorInsertt17d9b
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
TfR-mCh-DHFR
Plasmid#173013PurposeFor light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.DepositorInsertTfR-mCh-DHFR
UseTagsDHFR and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)
Plasmid#191141PurposePositive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codonDepositorInsertmCherry:s70587-W:EGFP
UseTagsExpressionMammalianMutationUAG>UGG (stop>Trp)PromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcsII-MECL1-flag
Plasmid#86774PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertimmunoproteasome beta subunit 2 (MECL1) (PSMB10 Human)
UseLentiviralTagsflagExpressionMammalianMutationPromoterunknownAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMJA219= pcDNA3.1V5-completeTRABV-CMVp
Plasmid#116878Purposemammalian expression vector containing TCR sequencesDepositorUseTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-HA-UBE3BΔHECT-IRES-Puro
Plasmid#107347PurposeExpresses HA tagged UBE3B deletion of HECT domain in mammalian cells.DepositorInsertUBE3BΔHECT (UBE3A Human)
UseLentiviralTagsHAExpressionMammalianMutationdeletion HECT domain (757-end)PromoterCMVAvailable sinceJune 26, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMJA285= pSin-TCRab-CMVp-Puro
Plasmid#116875Purposestable TCR expression in human T cellsDepositorUseLentiviralTagsExpressionMammalianMutationdeletion of EF1-a promotorPromoterCMV PromotorAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7b1(glu)
Plasmid#14673DepositorInsertMKK7b1(Glu) (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationSer-269Glu Thr-273GluPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
NSK024 SFFV-mCherry-SG(p53R248Q.s2)SG-EGFP (FLP-IN)
Plasmid#191169PurposeExpression of a sensor for p53 mutant R248Q in mammalian cells; mCherry marker, EGFP outputDepositorInsertmCherry:p53(R248Q).s2:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMJA289= pSin-EF2-TRAV10-P2A-TRBV25-Puro
Plasmid#116876Purposestable TCR expression in human T cellsDepositorUseLentiviralTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3-zz-p15_O
Plasmid#147110PurposeMammalian Expression of zz-p15DepositorInsertzz-p15 (NXT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV128-Rluc-CTE_O
Plasmid#147106PurposeMammalian Expression of Rluc-CTEDepositorInsertRluc-CTE
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
EK0498 SFFV-mCherry-SGc(ACTB.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191182PurposeExpression of EACTB sensor in mammalian cellsDepositorInsertmCherry:ACTB.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZL Hygro-NMYC TRF2 deltaB deltaM
Plasmid#16355DepositorInserthTRF2 (TERF2 Human)
UseRetroviralTagsMycExpressionMammalianMutationDeleted Basic Domain (aa1-44) and Myb Domain (aa4…PromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
-
pWZL Hygro-NFLAG TRF1
Plasmid#16277DepositorInserthTRF1 (TERF1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationaa2-439PromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only