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phU6-gRNA

Plasmid

#53188

Purpose

Expresses the S. pyogenes sgRNA from the human U6 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pSLCAR-CD19-28z

Plasmid

#135991

Purpose

Modular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Article

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-28-3z-P2A-EGFP (CD28, CD247 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a-short

Available since

June 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHR' CMV GFP

Plasmid

#14858

Depositor

Inder Verma

Article

Miyoshi et al Proc Natl Acad Sci U S A. 1997 Sep 16. 9

Insert

CMV-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-NONO

Plasmid

#127655

Purpose

Over-express HA-tagged human NONO

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

Insert

NONO (NONO Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-mSTING

Plasmid

#214157

Purpose

Transient expression of STING

Depositor

Nan Yan

Article

Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

Insert

STING (Sting1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pmScarlet-I3_C1

Plasmid

#189756

Purpose

C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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attB-puro donor.dna

Plasmid

#181923

Purpose

attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attB-EGFP-EF1α-PuroR-T2A-TagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLX_305_Ovalbumin

Plasmid

#184924

Purpose

Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

Depositor

Arlene Sharpe

Article

Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

Insert

Ovalbumin (OVAL Chicken)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Our vector has an A188T mutation that does not im…

Promoter

PGK

Available since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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gag-PE

Plasmid

#211371

Purpose

Packaging plasmid for v3 PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-PE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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gTBEv3

Plasmid

#220619

Purpose

vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Article

Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

Insert

hUNG2-nSpCas9

Use

Tags

Expression

Mammalian

Mutation

hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

Promoter

hU6, EF1a

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiFC-VC155

Plasmid

#22011

Purpose

Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

VC155

Use

Tags

HA

Expression

Mammalian

Mutation

Changed Alanine 206 to Lysine

Promoter

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV/D377Y-mPCSK9

Plasmid

#58376

Purpose

Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Article

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Insert

proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

D377Y

Promoter

HCRApoE/hAAT

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry

Plasmid

#197420

Purpose

Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

Cas9

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 WT

Plasmid

#69003

Purpose

mammalian expression of wild type human p53

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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PPRE X3-TK-luc

Plasmid

#1015

Purpose

PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

Depositor

Bruce Spiegelman

Article

Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

Insert

PPRE

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only

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EB3-tdTomato

Plasmid

#50708

Purpose

expresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

Depositor

Erik Dent

Article

Merriam et al J Neurosci. 2013 Oct 16;33(42):16471-82.

Insert

EB3 (MAPRE3 Human)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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Lenti-luciferase-P2A-Neo

Plasmid

#105621

Purpose

3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Luciferase

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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COM-epegRNA-Dnmt1

Plasmid

#211376

Purpose

Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

COM-epegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Human V5-IRF3-pcDNA3

Plasmid

#32713

Depositor

Saumen Sarkar

Article

Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

Insert

IRF3 (IRF3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

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Lck-mScarlet-I

Plasmid

#98821

Purpose

In vivo visualization of the plasma membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

Lck

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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