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Addgene
Showing: 7121 - 7140 of 69457 results

  1. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid
    #58477
    Purpose
    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Article
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed Arginine 62 to Aspartic Acid
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    58477-plasmid-map-sequence-id-90386

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  4. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

    Plasmid
    #58490
    Purpose
    Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage
    Depositor
    Adam Cohen
    Article
    Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3
    Insert
    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Changed Aspartic Acid 95 to Glutamine
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    58490-plasmid-map-sequence-id-90354

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  6. Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro

    Plasmid
    #58492
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    IRE1a (ERN1 Human)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    deleted amino acids P29 to D408 and bearing mutat…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    58492-plasmid-map-sequence-id-90361

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Showing: 7121 - 7140 of 69457 results