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Addgene
Showing: 6541 - 6560 of 69441 results

  8. pcDNA3.1/HisA/Serca1

    Plasmid
    #74402
    Purpose
    expression of SERCA in mammalian cells
    Depositor
    Robert Nakamoto
    Insert
    SERCA1 (ATP2A1 rabbit)
    Use
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    NcoI site destroyed (silent) T898
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  9. pdCas9-DNMT3A-PuroR_v2

    Plasmid
    #74407
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldoš
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    74407-plasmid-map-sequence-id-140317

    Enlarge

  10. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldoš
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

    Enlarge

  12. pBABE.is4-mOpn

    Plasmid
    #74410
    Purpose
    Expresses mouse Osteopontin from transcript tv4 in mammalian cells
    Depositor
    Georgios Stathopoulos
    Insert
    mouse Opn (Spp1 Mouse)
    Use
    Tags
    none
    Expression
    Mammalian
    Mutation
    Promoter
    MMLV
    Availability
    Academic Institutions and Nonprofits only
    74410-plasmid-map-sequence-id-142377

    Enlarge

  13. pBABE.is2-mOpn

    Plasmid
    #74411
    Purpose
    Expresses mouse Osteopontin from transcript tv2 in mammalian cells
    Depositor
    Georgios Stathopoulos
    Insert
    mouse Opn (Spp1 Mouse)
    Use
    Tags
    none
    Expression
    Mammalian
    Mutation
    Promoter
    MMLV
    Availability
    Academic Institutions and Nonprofits only
    74411-plasmid-map-sequence-id-142385

    Enlarge

  16. pRFP.mIKKε

    Plasmid
    #74414
    Purpose
    Expresses mouse RFP-IKKε in mammalian cells
    Depositor
    Georgios Stathopoulos
    Insert
    mouse IKKε (Ikbke Mouse)
    Use
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    74414-plasmid-map-sequence-id-140322

    Enlarge

  17. pCB6-EnvA

    Plasmid
    #74420
    Purpose
    Mammalian expression of the subgroup A avian leukosis-sarcoma envelope glycoprotein (EnvA)
    Depositor
    Judith White
    Article
    Gilbert et al J Virol. 1994 Sep;68(9):5623-8.
    Insert
    Env from ASLV-A
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
Showing: 6541 - 6560 of 69441 results