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Plasmid#142024PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertETV1 (ETV1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PEmax_AAVS1_knockin_HDR_donor
Plasmid#223000PurposeHDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPEmax
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-No-HaloTag7-mGFP-LC3-mRFP
Plasmid#184902PurposeStable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic fluxDepositorInsertLC3B
UseTagsHaloTag7, monomeric EGFP, and monomeric RFPExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-GFP
Plasmid#85556Purpose2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFPDepositorInsertKRAB-dCas9-IRES-GFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRPML1-YFP
Plasmid#18826DepositorInsertTRPML1 (MCOLN1 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA
Plasmid#154867PurposeFlp-dependent hM4D(Gi) DREADD-mCherryDepositorHas ServiceAAV Retrograde and AAV8InserthM4D(Gi)-mCherry
UseAAV; Flp-frtTagsmCherryExpressionMammalianMutationPromoterHuman Synapsin-1Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
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XLone-ETV2
Plasmid#135763Purposeexpresses human ETV2 upon doxycycline treatmentDepositorInsertETV2 (ETV2 Human)
UseTagsExpressionMammalianMutationnonePromoterTRE3GAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-NuMA
Plasmid#81029PurposeExpresses human NuMA with N-terminal EGFP tag in mammalian cellsDepositorInsertHomo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAF0034 Ef1a-Kp03-2a-EGFP
Plasmid#193461PurposeExpression of RecombinaseDepositorInsertKp03
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
EB3N-VVDfast-Halo-iLID
Plasmid#190166PurposeExpresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.DepositorInsertEB3N-VVDfast-Halo-iLID
UseTags-VVDfast-mClover3-iLIDExpressionMammalianMutation1-189 aa from EB3PromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2T-cmv-SpCas9-TadCBEd-V106W-BlastR
Plasmid#193849PurposeExpress TadCBEd-V106W in mammalian cells with BlastR selection, Tol2 integrationDepositorInsertTadCBEd-V106W
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_StrepII_TEV_PP2Ac
Plasmid#217477PurposeExpresses PP2Ac with a TEV cleavable N-terminal StrepII tagDepositorInsertPP2Ac
UseTagsStrepII, TEVExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cas9 sgRNA vector
Plasmid#68463PurposeCas9 sgRNA vector for cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab11a-7
Plasmid#55124PurposeLocalization: Ras GTPase, Excitation: 587, Emission: 610DepositorInsertRab11a (RAB11A Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT2/CMV-Luc/PGK-SB100X
Plasmid#192869PurposeCarries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.DepositorInsertsluc
SB100X
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
B2088_BTB-IDR-FRB
Plasmid#215101PurposeExpresses BCL6 BTB(1-129)-mCherry-FUS (IDR)-FRB in mammalian cellsDepositorInsertBTB-mCherry-FUS(IDR)-FRB
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-ZFYVE27-WPRE-UbC-mCherry
Plasmid#214470PurposeLentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-42:AAVS1-mTagRFPT-CAAX
Plasmid#107580PurposeHomology arms, promoter and linker-mTagRFPT-CAAX sequence for internal insertion of CAGGS-driven mTagRFPT-CAAX at the AAVS1 safe harbor location in human cellsDepositorInsertAAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX (AAVS1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMay 9, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
N174-MCS
Plasmid#81061PurposeLentiviral vector for constitutive gene expression.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only