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Plasmid#60526PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only
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ADORA2A-Tango
Plasmid#66210PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertADORA2A (ADORA2A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC12A9
Plasmid#131897PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC12A9 (SLC12A9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF0656
Plasmid#141653PurposeLentiviral vector for overexpressing the CHD3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCHD3 (CHD3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-InPAkt
Plasmid#184053PurposeLysosome-targeted FRET-based biosensor for monitoring 3-phosphoinositide dynamics in living cells.DepositorInsertLyso-InPAkt
UseTagsLysosome-associated membrane protein 1 (LAMP1).ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0301 SFFV-mCherry-SG(Bdnf.s1)SG-EGFP (FLP-IN)
Plasmid#191144PurposeExpression of Sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, EGFP output; 90 bp longDepositorInsertmCherry:Bdnf.s1:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-dV-IgG1/κ
Plasmid#52213PurposeHuman immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) coloniesDepositorInsertsHuman immunoglobulin gamma 1 constant region
Human immunoglobulin kappa constant region
UseTagsExpressionMammalianMutationDeleted Variable regionPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-3XKD/Y1699C
Plasmid#25367DepositorInsertLRRK2 (LRRK2 Human)
UseTags2XMycExpressionMammalianMutation"Kinase Dead" Lysine 1906 mutated to A…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-6013x
Plasmid#108654PurposeAn HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertRaichu-EV
UseLentiviralTagsXFPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRC-CMV-Rev1b
Plasmid#164443PurposeEncodes HIV-1 Rev accessory protein for pseudovirus productionDepositorInsertHIV-1 Rev
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF2238
Plasmid#142177PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGATA4 (GATA4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NHERF1-PDZ1-2
Plasmid#28294DepositorInsertNHERF1 (NHERF1 Human)
UseTagsFLAGExpressionMammalianMutationContains aa 1-298 (deletion of the EB domain aa 2…PromoterAvailable sinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
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piRFP670-N1
Plasmid#45457DepositorHas ServiceCloning Grade DNAInsertiRFP670
UseTagsExpressionMammalianMutationT2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…PromoterCMVAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast
Plasmid#205550PurposeExpress EPN24-MCP-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN24-MCP
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330.sgNf1.4
Plasmid#92028PurposepX330 sgNf1.4 for Nf1 deletionDepositorInsertsgNf1.4
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV566 empty vector
Plasmid#33349DepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-K1347A
Plasmid#25365DepositorInsertLRRK2 (LRRK2 Human)
UseTags2XMycExpressionMammalianMutationLysine 1347 mutated to AlaninePromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
MXS_BidirectionalCAG
Plasmid#78175PurposeBidirectional CAG promoter (compatible with the MXS Chaining Kit)DepositorInsertBidirectional CAG Promoter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133197
Plasmid#152383PurposeMammalian expression plasmid for SARS-CoV-2 helicaseDepositorInsertSARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits