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Plasmid#119764PurposeLentiviral plasmid for Mammalian ExpressionDepositorInsertAID-Dam-lamin B1
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC
Plasmid#125692PurposeGlobal labeling of inhibitory synapses (green fluorescence)DepositorInsertGephyrin.FingR-eGFP-CCR5TC
UseAAVTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-Tol2 transposase
Plasmid#158774PurposeExpression of Tol2 transposaseDepositorInsertTol2 tranposase
UseTagsExpressionMammalianMutationTol2 codon usage frequency well suitable for muri…PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST pcDNA5-FRT/TO-eGFP-Rif1
Plasmid#52506Purposemammalian expression vector of Rif1 siRNA resistantDepositorInsertRIF1 (RIF1 Human)
UseTagseGFPExpressionMammalianMutationsiRNA resistant sequence: ATAGTACPromoterAvailable sinceJune 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUCmini-iCAP-AAV9.452sub.LUNG1
Plasmid#184592Purposenon-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system for mouse lung transduction after intravenous injectionDepositorInsertSynthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
UseAAVTagsExpressionMammalianMutation7 amino acid substitution between VP1 452 and VP1…Promoterp41Available sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMH-MYC
Plasmid#101765PurposeGateway-compatible destination vector for mammalian expression of N-terminal Myc-tag fusion proteinDepositorTypeEmpty backboneUseTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-CAPN1
Plasmid#60941PurposeExpresses calpain1 in mammalian cellsDepositorInsertCalpain1 (CAPN1 Human)
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt1
Plasmid#184502PurposeMammalian expression of Synaptotagmin 1 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 1 (Syt1 Rat)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.pU1a-SpCas9
Plasmid#121507PurposeExpresses codon-optimized SpCas9 in mammalian cells.DepositorInsertSpCas9
UseTagsHAExpressionMammalianMutationPromoterU1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF.myc.ER-E2-Crimson
Plasmid#38770PurposeIn vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)DepositorInsertE2-Crimson
UseTagsER retention signal (KDEL), ER signal sequence (M…ExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPPAT-EGFP
Plasmid#99105PurposeExpresses human PPAT-EGFP fusion in mammalian cellsDepositorInsertPPAT (PPAT Human)
UseTagsEGFP (enhanced green fluorescent protein)ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(MapErk)dTomato
Plasmid#200113PurposeFluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertMapErk sequence in minimal promoter
UseTagsExpressionMammalianMutationhighly modified sequencePromoter5 copies of designed MapErk sequence, no other pr…Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCh-Cry2olig
Plasmid#101222PurposeCry2olig(E490G)DepositorInsertCry2olig
UseLentiviralTagsExpressionMammalianMutationE490GPromoterSFFVAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3
Plasmid#165430PurposeExpresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.DepositorInsertFHS-paCaMKII(SD)
UseAAVTagsFHS(Flag, HisX6, Strep) tagExpressionMammalianMutationC450A, L514K, G528A, L531E, N538E: Light-insensit…PromoterCaMKII0.4Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pDest eGFP MBNL1 42 kDa
Plasmid#61277PurposeMammalian expression of EGFP-tagged human MBNL1DepositorInsertMBNL1 (MBNL1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1.Gαq-LgB97
Plasmid#134360PurposeNanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.DepositorInsertGαq-LgB97 (GNAQ Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF1637
Plasmid#143830PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHOXC12 (HOXC12 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-B19L
Plasmid#59922PurposeExpression vector for Rabies virus SAD B19 RNA dependent RNA polymerase geneDepositorInsertRabies virus RNA dependent RNA polymerase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only