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Plasmid#72666PurposeLentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)DepositorInserth7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mito NAD+ biosensor
Plasmid#186791PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondriaDepositorInsertmito NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
DBD (N208AH212R) Foxo1-WT pDNR-CMV
Plasmid#17555DepositorInsertFoxo1-WT-DBD (Foxo1 Mouse)
UseTagsFLAGExpressionMammalianMutationDNA binding-deficientChanged N208A H212RPromoterCMVAvailable sinceOct. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
FLAG-TRAF6-wt
Plasmid#21624DepositorInsertTNF receptor-associated factor 6 (Traf6 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMOS003: GCaMPer calcium sensor (endoplasmic reticulum)
Plasmid#163047PurposeGCaMPer calcium sensor (endoplasmic reticulum)DepositorInsertGCaMPer
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-RQ/TG
Plasmid#25369DepositorInsertLRRK2 (LRRK2 Human)
UseTags2XMycExpressionMammalianMutationThreonine 1343 mutated to Glycine Arginine 139…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
MTK3_010
Plasmid#123725PurposeEncodes Tet-On 3G transactivator protein as a Type 3 part to be used in the MTK systemDepositorInsertTetOn3G
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN-c-JUN
Plasmid#102758PurposeExpression of c-JunDepositorInsertc-Jun (JUN Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES
Plasmid#200402PurposeEntry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detectionDepositorInsertClosed-loop DART VADAR sensor entry cassette
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab4B
Plasmid#61801PurposeHuman Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expressionDepositorInsertRab4B (RAB4B Human)
UseTagsGFPExpressionMammalianMutationNonePromoterCMVAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-Ago2
Plasmid#215841PurposeExpression of AGO2DepositorInsertHuman AGO2 (AGO2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF3426
Plasmid#144902PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF350 (ZNF350 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-INPP4B-mCherry
Plasmid#184055PurposeLysosome targeted inositol polyphosphate-4-phosphatase type II B; tagged with mCherry.DepositorUseTagsLysosome-associated membrane protein 1 (LAMP1). a…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab5a-7
Plasmid#55126PurposeLocalization: Ras GTPase, Excitation: 587, Emission: 610DepositorInsertRab5a
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-EKAR4
Plasmid#205513PurposeGenetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.DepositorInsertendo-EKAR4
UseTags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG_iPE-N
Plasmid#214734Purposeencodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoterDepositorInsertCAG_iPE-N_bGH
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMAL
Plasmid#161785PurposeGateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and GFP.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterBidirectional minhCMV and SFFVAvailable sinceDec. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
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V5-APEX2-LMNA
Plasmid#129275PurposeExpression of APEX2 at nuclear laminaDepositorInsertV5-APEX2-LMNA
UseLentiviralTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-surfaceHA-IRES-Puro
Plasmid#207321PurposeLentiviral expression of synthetic HA surface receptor in Mammalian cellsDepositorInsertHAtag-Myctag-PDGFRB-TM-IRES-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alpha_short (EFS)Available sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only