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Plasmid#185912PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpRY-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-LaminA-C-18
Plasmid#55068PurposeLocalization: Nuclear Envelope, Excitation: 587, Emission: 610DepositorInsertLamin A (LMNA Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-GFP1-10
Plasmid#68715PurposeSplit GFP assayDepositorInsertGFP1-10
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20_mGFP
Plasmid#190027PurposeDoxycycline-inducible lentiviral vector for expression of ORF with C-terminal mGFPDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBtet rtTA G72V SE Δsplice GP Luc
Plasmid#194328PurposeTetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightnessDepositorInsertLuciferase
UseLuciferaseTagsExpressionMammalianMutationTet-responsive promotor related mutations: G72V i…PromoterTight tet-responsive promotorAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
PG13-luc (wt p53 binding sites)
Plasmid#16442PurposeLuciferase reporter containing 13 copies of the p53-binding consensus sequenceDepositorInsertp53-binding site (TP53 )
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
3xnls-mTurquoise2
Plasmid#98817PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorArticleInsert3xNLS
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
iRFP-P4M-SidM
Plasmid#51470PurposeBiosensor for PtdIns4PDepositorInsertP4M-SidM(546-647)
UseTagsiRFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Soma-jGCaMP8s
Plasmid#169256PurposeAAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8s.DepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertSoma-jGCaMP8s
UseAAVTags6xHis-tagExpressionMammalianMutationPromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIRT3 Flag
Plasmid#13814PurposeMammalian expression of human SIRT3 with flag tagDepositorInsertSIRT3 (SIRT3 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDmelOR
Plasmid#126476PurposeVector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intronDepositorInsertDmelOrco (Orco Fly)
UseTagsMycExpressionMammalianMutationcodon optimization for H. sapiens; includes a rec…PromoterCMVAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.Twitch2B.WPRE.SV40
Plasmid#100039PurposeAAV expression of FLEXed Twitch2B driven by CAG promoter. Twitch 2B is a fluorescent reporter for calcium signalingDepositorInsertTwitch2B
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 mCherry
Plasmid#128073Purposepuro exchanged to mCherryDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationnoPromoterAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL2-NOS2Promoter-Luciferase
Plasmid#19296DepositorInsertNOS2 Promoter (Nos2 Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mtagBFP
Plasmid#89685PurposepCAG promoter driving mtagBFPDepositorInsertmtagBFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC1-HyPer-3
Plasmid#42131DepositorInsertHYPER 3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMVβ-p300-myc
Plasmid#30489PurposeMammalian expression of human p300 with myc tagDepositorInsertp300 (EP300 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pB80-SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)
Plasmid#174643PurposeOptogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transportDepositorInsertSSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)
UseTagsSSPB(nano)-GFPExpressionMammalianMutationEGFP: Met1Del; ppKin14VIb(aa861-1321)PromoterChicken beta-actinAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC12A9
Plasmid#131897PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC12A9 (SLC12A9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-Lyso-InPAkt
Plasmid#184053PurposeLysosome-targeted FRET-based biosensor for monitoring 3-phosphoinositide dynamics in living cells.DepositorInsertLyso-InPAkt
UseTagsLysosome-associated membrane protein 1 (LAMP1).ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only