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Plasmid#214066PurposeFluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)
Plasmid#133801PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA4
UseTagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
GPR65-Tango
Plasmid#66370PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR65 (GPR65 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 mSynCAM3
Plasmid#115498Purposeexpression of mouse full-length SynCAM 3DepositorInsertCadm3 (Cadm3 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)
Plasmid#225752PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (RFP)
Plasmid#80923PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOS WT-GL3
Plasmid#11983PurposeReporter gene with mouse c-fos promoter. Induced by serum and many growth factors in quiescent cells.DepositorInsertc-fos promoter (Fos Mouse)
UseTagsLucExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)
Plasmid#128604PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-1P-WPRE
Plasmid#202611PurposeDouble floxed genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)DepositorInsertJEDI-1P
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL2783 (pTnsC-VP64_Vch)
Plasmid#200896PurposeExpresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.DepositorInsertVchTnsC-VP64
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_G3BP1
Plasmid#127106DepositorInsertG3BP1 (G3BP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
AZ64_pE.DonorCLYBL.TS
Plasmid#199228PurposeDonor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locusDepositorInsertExpression unit for EGFP reporter
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
shCD44-2 pRRL
Plasmid#19123DepositorInsertsmall hairpin RNA against CD44 (CD44 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
Desmoglein2-mCherry
Plasmid#36991DepositorInsertDsg2 (DSG2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-M3nls
Plasmid#155366PurposeTargeted m6A RNA methylation in mammalian cellsDepositorInsertdCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
UseCRISPRTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133APromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ (M)-H2A-HT
Plasmid#82517PurposeLentivirus vector. Expresses H2A-Halotag fusion proteins in mammalian cells.DepositorInsertHistone H2A
UseLentiviralTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-JMJD2A-Hygro
Plasmid#104410Purposetransient expression of dCas9-JMJD2A fusion proteinDepositorInsertJMJD2A (KDM4A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Ace-mNeon2
Plasmid#195526PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoterDepositorInsertAce-mNeon2
UseAAVTagsExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterCaMKIIAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
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